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Detection of head and neck cancer by spraying cancer-specific fluorescent probe

Research Project

Project/Area Number 26461043
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionHokkaido University

Principal Investigator

Ono Shoko  北海道大学, 大学病院, 助教 (60431376)

Research Collaborator Urano Yasuteru  東京大学, 大学院医学研究科, 教授 (20292956)
Shimizu Yuichi  北海道大学, 北海道大学病院, 准教授 (90333608)
Ohnishi Shunsuke  北海道大学, 大学院医学研究科, 准教授 (10443475)
Hatanaka Kanako  北海道大学, 北海道大学病院, 特任講師 (10399834)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords頭頸部がん / 蛍光イメージング
Outline of Final Research Achievements

γ-glutamyltranspeptidase (GGT), a cell surface enzyme, is overexpressed in several cancers, and it has been reported that γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), a fluorescent targeting agent which can be enzymatically activated and becomes fluorescent after cleavage of a GGT-specific sequence. We investigated whether early HNSCC can be detected by applying gGlu-HMRG to clinical samples.
All four gGlu-HMRG-applied cell lines emitted green fluorescence. Immunohistological examination demonstrated that GGT was highly expressed in HNSCC of the recent three ESD cases but barely expressed in the normal mucosa. Fluorescence imaging showed that iodine-voiding lesions became fluorescent within a few minutes after application of gGlu-HMRG in all eight resected cases. Tumor ROI fluorescence intensity was significantly higher than in the normal mucosa five minutes after gGlu-HMRG application. Fluorescence imaging with gGlu-HMRG would be useful for early detection of HNSCC.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Fluorescent imaging of superficial head and neck squamous cell carcinoma using a γ-glutamyltranspeptidase-activated targeting agent: a pilot study.2016

    • Author(s)
      Mizushima T, Ohnishi S, Shimizu Y, Hatanaka Y, Hatanaka KC, Hosono H, Kubota Y, Natsuizaka M, Kamiya M, Ono S, Homma A, Kato M, Sakamoto N, Urano Y
    • Journal Title

      BMC Cancer

      Volume: 16 Issue: 1 Pages: 411-411

    • DOI

      10.1186/s12885-016-2421-z

    • NAID

      120005773989

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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