Molecular basis for clinical cancer stem cell targeted photodynamic therapy

• Project/Area Number: 26461045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tottori University (2015-2016); Nagasaki University (2014)
• Principal Investigator: Isomoto Hajime 鳥取大学, 医学部, 教授 (90322304)
• Co-Investigator(Kenkyū-buntansha): 七島 篤志 宮崎大学, 医学部, 教授 (60380838)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 光線力学療法 / 光線力学的療法 / 食道癌 / 化学放射線療法 / 扁平上皮癌 / タラポルフィンナトリウム / タラポルフィリンナトリウム

Outline of Final Research Achievements

We performed cellular experiments to explore molecular basis for squamous cell carcinoma stem cell based photodynamic therapy (PDT) using talaporphin sodium. The cells with igher levels of CD44 variant are to be targeted via cistine transporter-glutathione in which cells are to be resistant to oxidative stress. For this aim, we performed MTS and glutathione-generation assay in the setting of PDT under sulphasarazopirine (SASP) administration. The results showed SASP had nominal additional effects on PDT.

Research Products

• [Presentation] The molecular basis of Laser-based Photodynamic Endoscopic diagnosis for gastric tumors (2017)
  • Author(s): Hiroki Kirumi, Kumi Ogihara, Hajime Isomoto
  • Organizer: DDW2017
  • Place of Presentation: McCormick Place, Chicago, IL, USA
  • Year and Date: 2017-05-06
  • Int'l Joint Research
• [Presentation] 胃癌に対するレーザー光線力学的診断の分子基盤 (2017)
  • Author(s): 菓 裕貴, 荻原久美, 磯本 一
  • Organizer: 日本消化管学会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2017-02-17