Immunohistochemical identification of Propionibacterium acnes in granuloma and inflammatory cells of myocardial tissues obtained from cardiac sarcoidosis patients
| Project/Area Number |
26461051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
絹川 真太郎 北海道大学, 医学研究科, 講師 (60399871)
筒井 裕之 九州大学, 医学研究院, 教授 (70264017)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 心サルコイドーシス / P.acnes / 心筋生検 / アクネ菌 / 免疫学的染色 |
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| Outline of Final Research Achievements |
In cardiac sarcoidosis、early diagnosis and intervention are essential, pathological diagnosis is limited. Propionibacterium acnes is a implicated aetiologic agent of sarcoidosis We examined myocardial tissues obtained from patients with CS (n=26; CS-group), myocarditis (n=15; M-group), or other cardiomyopathies (n=39; CM-group) using immunohistochemistry (IHC) with a P. acnes antibody. We found granulomas in 16 (62%) CS-group samples. Massive (>13 inflammatory cells) and minimal (<12) inflammatory foci,were detected in 16 (62%) and 11 (42%) of the CS-group samples, 10 (67%) and 10 (67%) of the M-group samples, and 1 (3%) and 18 (46%) of the CM-group samples. P. acnes-positive reactivity in granulomas, massive and minimal inflammatory foci were detected in 10 (63%), 10 (63%), and 8 (73%) of the CS-group samples, in none of the other samples. IHC detection of P. acnes in inflammatory foci of myocardial samples without granulomas may be useful for differentiating sarcoidosis from others.
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Report
(4 results)
Research Products
(10 results)
