| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
miR-33a is located in the intron of SREBP2, which is a main transcriptional factor that regulates cholesterol homeostasis. On the other hand, miR-33b is located in the intron of SREBP1, which regulates fatty acid homeostasis in human. To elucidate the function of miR-33a in various organs or cells, we developed miR-33a conditional KO mice. When miR-33a was ablated in fibroblast cells, cardiac fibrosis was inhibited under pressure-overload condition via modulation of lipid rafts. To elucidate the function of miR-33b in vivo, we developed miR-33b knock-in mice, which have human miR-33b sequence in the same intron of SREBP1 as human. These mice showed reduced expression of ABCA1 and decreased levels of serum HDL-C. These results indicate that miR-33a/b have multiple functions in regulating lipid homeostasis.
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