Neutrophil Extracelluar Traps in Severe influenza-elated pneumonia

• Project/Area Number: 26461158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Tohoku Medical and Pharmaceutical University (2015-2016); Osaka University (2014)
• Principal Investigator: SEKI Masafumi 東北医科薬科大学, 医学部, 教授 (80432970)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: インフルエンザ / 肺炎 / サイトカインストーム / 好中球 / 敗血症 / ネッツ / ワクチン / 耐性菌 / NETs / マクロライド系薬 / MRSA / 真菌症 / 誤嚥性肺炎 / ヒトメタニューモウイルス / 真菌

Outline of Final Research Achievements

The aim of this study is that examination whether excessive immunological reaction, including NETs (Neutrophil Extracellular Traps) make the damage to host and patients although these regularly could control the infectious pathogens.
We reported these possible cases including severe influenza-related pneumonia, and analyzed mice models of sepsis and influenza infection. In addition, we investigated the diagnosis method including biomarkers (eg. procalcitonin) in clinical fields.

Research Products

• [Journal Article] Association of influenza with severe pneumonia/empyema in the community, hospital, and healthcare-associated setting. (2016)
  • Author(s): Seki M, Fuke R, Oikawa N, Hariu M, Watanabe Y,
  • Journal Title: Resp Med Case Rep. Volume: 19 Pages: 1-4
  • DOI: 10.1016/j.rmcr.2016.05.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Chronic Respiratory <i>Pasteurella multocida</i> Infection Is Well-Controlled by Long-Term Macrolide Therapy (2016)
  • Author(s): Seki M, Sakata T, Toyokawa M, Nishi I, Tachibana I, Tomono K, Kumanogoh A,.
  • Journal Title: Internal Medicine Volume: 55 Issue: 3 Pages: 307-310
  • DOI: 10.2169/internalmedicine.55.4929
  • NAID: 130005122596
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ability of procalcitonin to diagnose bacterial infection and the bacteria types compared with blood culture findings. (2016)
  • Author(s): Watanabe Y, Oikawa N, Hariu M, Fuke R, Seki M,
  • Journal Title: Inter J Gen Med. Volume: 9 Pages: 325-331
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Toll-like Receptor 4 Agonistic Antibody Promotes Host Defense against Chronic Pseudomonas aeruginosa Lung Infection in Mice. (2016)
  • Author(s): 4)Nakamura S, Iwanaga N, Seki M, Fukudome K, Oshima K, Miyazaki T, Izumikawa K, Yanagihara K, Miyazaki Y, Mukae H, Kohno S.
  • Journal Title: Infect Immun. Volume: 84 Issue: 7 Pages: 1986-1993
  • DOI: 10.1128/iai.01384-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] oinfection of influenza virus and group A Streptococcus in an adult suspected with infectious mononucleosis by Epstein-Barr virus infection. (2016)
  • Author(s): 5)Seki M, Oikawa N, Hariu M, Watanabe Y,
  • Journal Title: Global J Clin Virol. Volume: 1 Pages: 5-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] PCR based active surveillance of Methicillin-Resistant Staphylococcus aureus (MRSA) in emergency department patients. (2015)
  • Author(s): Seki M, Takahashi H, Yamamoto N, Hamaguchi S, Ojima M, Hirose T, Yoshiya K, Ogura H, Shimazu T, Tomono K.
  • Journal Title: Infection and Drug Resistance Volume: 8 Pages: 113-118
  • DOI: 10.2147/idr.s80123
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clinical Specimen-Direct LAMP: A Useful Tool for the Surveillance of blaOXA-23-Positive Carbapenem-Resistant Acinetobacter baumannii.. (2015)
  • Author(s): Yamamoto N, Akeda Y, Hamaguchi S, Santanirand P, Kedidsin A, Seki M, Phang W, Malathum K, Oishi K, Tomono K.
  • Journal Title: PLoS One Volume: 10 Issue: 7 Pages: e0133204-e0133204
  • DOI: 10.1371/journal.pone.0133204
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Usefulness of a phage-open reading frame typing (POT) kit for rapid identification of Methicillin-Resistant Staphylococcus aureus (MRSA) transmission in a tertiary hospital (2015)
  • Author(s): Takahashi H, Seki M, Yamamoto N, Hamaguchi S, Ojima M, Hirose T, Yoshiya K, Toyokawa M, Nishi I, Ogura H, Shimazu T, Tomono K.
  • Journal Title: Infection and Drug Resistance Volume: 8 Pages: 107-111
  • DOI: 10.2147/idr.s83509
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Invasive pulmonary aspergillosis due to Aspergillus lentulus: Successful treatment of a liver transplant patient. (2015)
  • Author(s): Yoshida H, Seki M, Umeyama T, Urai M, Kinjo Y, Nishi I, Toyokawa M, Kaneko Y, Ohno H, Miyazaki Y, Tomono K.
  • Journal Title: J Infect Chemother Volume: epub Issue: 6 Pages: 479-481
  • DOI: 10.1016/j.jiac.2015.02.010
  • Peer Reviewed / Open Access
• [Journal Article] The origin of circulating free DNA in sepsis: Analysis of CLP mouse model. (2015)
  • Author(s): Hamaguchi S, Akeda Y, Yamamoto N, Seki M, Oishi K, Tomono K
  • Journal Title: Mediators of Inflammation Volume: 2015 Issue: 1 Pages: 614518-614527
  • DOI: 10.1155/2015/614518
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] (2014)
  • Author(s): Seki M., Ohno H., Gotoh K., Motooka D., Nakamura S., Iida T., Miyazaki Y., Tomono K.
  • Journal Title: IDCases Volume: 1 Issue: 1 Pages: 5-8
  • DOI: 10.1016/j.idcr.2014.01.001
  • Peer Reviewed / Open Access
• [Journal Article] Severe respiratory failure due to co-infection with human metapneumovirus and Streptococcus pneumoniae. (2014)
  • Author(s): Seki M, Yoshida H, Gotoh K, Hamada N,Motooka D, Nakamura S, Yamamoto N, Hamaguchi S, Akeda Y, Watanabe H, Iida T, and Tomono K.
  • Journal Title: Respiratory Medicine Case Reports Volume: 12 Pages: 13-15
  • DOI: 10.1016/j.rmcr.2013.12.007
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of combination therapy with oseltamivir phosphate and azithromycin for influenza. (2014)
  • Author(s): Kakeya H, Seki M, Izumikawa K, Kosai K, Morinaga Y, Kurihara S, Nakamura S, Imamura Y, Miyazaki T, tsukamoto M, Yanagihara K, Tashiro T, Kohno S.
  • Journal Title: PLoS One Volume: 9 Issue: 3 Pages: e91293-e91293
  • DOI: 10.1371/journal.pone.0091293
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Presence of neutrophil extracellular traps and citrullinated histone h3 in the bloodstream of critically ill patients (2014)
  • Author(s): Hirose T, Hamaguchi S, Matsumoto N, et a
  • Journal Title: PLoS One Volume: Nov 13;9(11) Issue: 11 Pages: e111755-e111755
  • DOI: 10.1371/journal.pone.0111755
  • NAID: 120006986309
  • Peer Reviewed / Open Access
• [Journal Article] Neutrophil extracellular traps in bronchial aspirates: a quantitative analysis (2014)
  • Author(s): Hamaguchi S., et.al.
  • Journal Title: European Respiratory Journal Volume: 印刷中 Issue: 6 Pages: 1709-18
  • DOI: 10.1183/09031936.00139813
  • Peer Reviewed / Open Access
• [Presentation] 多職種連携による感染対策の現状と今後～臨床現場が求める微生物検査と抗菌薬適正使用支援～ (2016)
  • Author(s): 関 雅文
  • Organizer: 感染症学会東日本総会 シンポジウム
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2016-10-26
  • Invited
• [Presentation] 呼吸器感染症の新世代への課題 臨床医の立場から～これまでのガイドライン作成でわかったこと、よかったこと～ (2016)
  • Author(s): 関 雅文
  • Organizer: 感染症学会東日本総会 特別企画
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2016-10-26
  • Invited
• [Presentation] インフルエンザ診療の最近の動向 (2016)
  • Author(s): 関 雅文
  • Organizer: 日本感染症学会総会 ランチョンセミナー
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-04-15
  • Invited
• [Presentation] 重症感染症にどう対応するか？ (2016)
  • Author(s): 関 雅文
  • Organizer: 日本感染症学会総会 イブニングセミナー
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-04-15
  • Invited
• [Presentation] 高齢者における呼吸器感染症～ウイルス感染症 (2016)
  • Author(s): 関 雅文
  • Organizer: 日本呼吸器学会総会 ICD講習会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-04-08
  • Invited
• [Presentation] 肺炎・感染症の重症化にどう対応するか？～ワクチンを中心に～ (2015)
  • Author(s): 関 雅文
  • Organizer: 日本化学療法学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 明日から役立つMRSA感染症治療に関する最新知識 (2015)
  • Author(s): 関 雅文
  • Organizer: 日本化学療法学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 重症感染症の病態把握と検査の重要性 (2015)
  • Author(s): 関 雅文
  • Organizer: 日本集中治療医学会東北地方会
  • Place of Presentation: 福島
  • Year and Date: 2015-05-23
  • Invited
• [Presentation] インフルエンザと関連する肺炎のマネージメントについて (2015)
  • Author(s): 関 雅文
  • Organizer: 日本呼吸器学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-04-17
  • Invited
• [Presentation] 誤嚥性肺炎に対する集学的アプローチ (2015)
  • Author(s): 関 雅文
  • Organizer: 日本呼吸器学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-04-17
  • Invited
• [Presentation] The Pathophysiological Comparison of Secondary Pneumococcal Pneumonia (2014)
  • Author(s): Seki M, Yamamoto N, Akeda Y, Hamaguchi S, Oishi K, Tomono K
  • Organizer: Asian Pacific Respiratory Siciety, General Meeting
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2014-11-14 – 2014-11-17
  • Invited
• [Presentation] ICTに必要なPK/PD理論と臨床効果 (2014)
  • Author(s): 関 雅文
  • Organizer: 西日本・中日本感染症・化学療法学会
  • Place of Presentation: 岡山
  • Year and Date: 2014-10-23 – 2014-10-25
  • Invited
• [Presentation] MRSA感染症診療の基本的考え方と実際 (2014)
  • Author(s): 関 雅文
  • Organizer: 日本感染症学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-06-18 – 2014-06-20
  • Invited
• [Presentation] 肺炎治療のファーストラインは？～ペニシリン系薬と他剤との使い分け～ (2014)
  • Author(s): 関 雅文
  • Organizer: 日本呼吸器学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-04-25 – 2014-04-27
  • Invited
• [Presentation] 抗菌薬選択とde-escalationの実際 (2014)
  • Author(s): 関 雅文
  • Organizer: 日本呼吸器学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-04-25 – 2014-04-27
  • Invited
• [Book] 抗菌薬おさらい帳 (2016)
  • Author(s): 関 雅文ら
  • Total Pages: 228
  • Publisher: じほう
• [Book] 医学と薬学 (2015)
  • Author(s): 関 雅文
  • Total Pages: 5
  • Publisher: 自然科学社
• [Book] 内科 (2015)
  • Author(s): 関 雅文
  • Total Pages: 5
  • Publisher: 南江堂
• [Book] 呼吸器内科 (2015)
  • Author(s): 関 雅文
  • Total Pages: 4
  • Publisher: 科学評論社
• [Book] Ｍｅｄｉｃｎａ (2015)
  • Author(s): 関 雅文
  • Total Pages: 3
  • Publisher: 医学書院
• [Book] 感染制御学 (2015)
  • Author(s): 関 雅文
  • Total Pages: 7
  • Publisher: へるす出版