| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We examined the potential for developing vaccines mediated by dendritic cells pulsed with P.aeruginosa pili protein or its peptides. Since we lost a large amount of the pili protein in the process removing LPS contamination, it was difficult to obtain enough quantity of pili protein for pulsing dendritic cells. Peptide (pili303) corresponding to the part from 94th to 143rd amino acid residues of pili protein of P.aeruginosa were synthesized. After dendritic cells pulsed with the peptide were co-cultured with the naive T-cells obtained from mouse spleens, the culture medium was harvested, and IFN-gamma and IL-10 were examined using ELISA. The naive T cells exposed to the peptide-pulsed dendritic cells produced a larger amount of IFN-gamma and IL-10 compared to those of the other peptide (pili6: 101st -120th amino acid of the pili protein). These results suggest that the antigenic presentation performed strongly by the peptide pili303-pulsed dendritic cells activates naive T cells.
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