Epitope mapping of autoantibody in autoimmune pulmonary alveolar proteinosis
| Project/Area Number |
26461169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Aichi Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高橋 歩 愛知医科大学, 医学部, 講師 (30374292)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 自己免疫性肺胞蛋白症 / 自己抗体 / 顆粒球マクロファージコロニー刺激因子 / エピトープマッピング / 表面プラズモン共鳴法 / GM-CSF / 表面プラズモンン共鳴 / SPR / 肺胞蛋白症 |
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| Outline of Final Research Achievements |
Autoimmune pulmonary alveolar proteinosis is caused by the production of autoantibody against GM-CSF that support maturation and proliferation of alveolar macrophages. We made an attempt of epitope mapping for serum antibodies to elucidate the site of GM-CSF molecules to which antibodies preferentially bind. No significant difference was observed by ELISA among individual peptides derived from GM-CSF or between patients and healthy volunteers. We could not establish reliable method to detect specific binding signals by surface plasmon resonance method. Thus, we have not succeeded in mapping epitopes.
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Report
(4 results)
Research Products
(7 results)
