Epitope mapping of autoantibody in autoimmune pulmonary alveolar proteinosis

• Project/Area Number: 26461169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Aichi Medical University
• Principal Investigator: Yamaguchi Etsuro 愛知医科大学, 医学部, 教授 (10201831)
• Co-Investigator(Kenkyū-buntansha): 高橋 歩 愛知医科大学, 医学部, 講師 (30374292)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 自己免疫性肺胞蛋白症 / 自己抗体 / 顆粒球マクロファージコロニー刺激因子 / エピトープマッピング / 表面プラズモン共鳴法 / GM-CSF / 表面プラズモンン共鳴 / SPR / 肺胞蛋白症

Outline of Final Research Achievements

Autoimmune pulmonary alveolar proteinosis is caused by the production of autoantibody against GM-CSF that support maturation and proliferation of alveolar macrophages. We made an attempt of epitope mapping for serum antibodies to elucidate the site of GM-CSF molecules to which antibodies preferentially bind. No significant difference was observed by ELISA among individual peptides derived from GM-CSF or between patients and healthy volunteers. We could not establish reliable method to detect specific binding signals by surface plasmon resonance method. Thus, we have not succeeded in mapping epitopes.

Research Products

• [Journal Article] A case of autoimmune pulmonary alveolar proteinosis with fluctuating lung shadows in parallel with cigarette smoke burden. (2017)
  • Author(s): Takahashi A, Yamaguchi E, et al
  • Journal Title: Sarcoidosis vasculitis and diffuse lung disease Volume: 34
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Two cases of autoimmune pulmonary alveolar proteinosis with rheumatoid arthritis (2017)
  • Author(s): Ito S, Wakahara K, Kojima T, Takahashi N, Nishiwaki, K, Yamaguchi E, and Hasegawa Y.
  • Journal Title: Allergology International Volume: 66 Issue: 3 Pages: 507-509
  • DOI: 10.1016/j.alit.2017.02.002
  • NAID: 130005858973
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Presentation] The cut-off value of serum KL-6 level for discrimination of autoimmune pulmonary alveolar proteinosis and interstitial pneumonia. (2016)
  • Author(s): Yamaguchi E, Takahashi A, et al.
  • Organizer: 12st Congress of Asian Pacific Society of Respirology
  • Place of Presentation: Bangkok, Thailand.
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] 自己免疫性肺胞蛋白症における血清抗GM-CSF IgG濃度の臨床的意義：発症閾値と改善閾値に関する検討 (2015)
  • Author(s): 髙橋 歩、山口悦郎
  • Organizer: 日本サーファクタント・界面医学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-10-31
• [Presentation] 不十分な全肺洗浄後に陰影が著明に改善した自己免疫性肺胞蛋白症の１例 (2015)
  • Author(s): 小坂顕司、山口悦郎他
  • Organizer: 第125回日本呼吸器学会東海地方会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-06-13
• [Presentation] Temporal Changes of the Serum Levels of Autoantibodies Against Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in Natural Clinical Course of Autoimmune Pulmonary Alveolar Proteinosis (APAP) (2015)
  • Author(s): Ayumu Takahashi, Etsuro Yamaguchi, et al.
  • Organizer: American Thoracic Society 2015
  • Place of Presentation: Denver
  • Year and Date: 2015-05-19
• [Presentation] APAP患者自己抗体のエピトープマッピング (2014)
  • Author(s): 髙橋 歩
  • Organizer: 肺胞蛋白症、遺伝性間質性肺炎に関する研究班 平成２６年度第２回班会議
  • Place of Presentation: 品川インターシティ
  • Year and Date: 2014-12-14