Effect of iron deficiency on animal model of pulmonary emphysema

• Project/Area Number: 26461177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Yamagata University
• Principal Investigator: SHIBATA Yoko 山形大学, 医学部, 講師 (60333978)
• Co-Investigator(Renkei-kenkyūsha): KUROTANI Reiko 山形大学, 大学院理工学研究科, 准教授 (00453043) ; ABE Shuichi 山形大学, 医学部, 非常勤講師 (40400543) ; INOUE Sumito 山形大学, 医学部, 助教 (70466621)
• Research Collaborator: SATO Masamichi ; SATO Kento
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 慢性閉塞性肺疾患 / 肺気腫 / 鉄 / たばこ / 鉄欠乏 / 喫煙 / ＣＯＰＤ / 血清鉄 / COPD

Outline of Final Research Achievements

Serum iron is reportedly related to smoking tolerance. It is also known that low serum iron levels are associated with rapid decline in respiratory function due to smoking. In this study, we investigated the mechanism why low level of serum iron leads to a decline in respiratory function in the smoke-exposed mouse emphysema model. After administering an iron deficient diet to mice, smoking exposure was carried out on the mice for two weeks using a smoking exposure apparatus. In bronchoalveolar lavage analysis, significant accumulation of alveolar macrophages was observed in the iron deficient food intake group as compared with the normal food ingestion group. Next, after the mice in both groups were exposed to smoking for 8 weeks, respiratory function tests and pathological examinations were performed to evaluate emphysema formation. Emphysema formation progressed in the iron deficient diet group compared to the normal diet group.

Research Products

• [Journal Article] 血清鉄低値は呼吸機能低下の原因なのか (2017)
  • Author(s): 柴田陽光、佐藤建人、井上純人
  • Journal Title: 別冊BIO Clinica 呼吸器疾患と慢性炎症 Volume: 6 Pages: 101-105
• [Journal Article] MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression. (2017)
  • Author(s): Nemoto T, Shibata Y, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Sato M, Sato K, Nakano H, Abe S, Nishiwaki M, Kubota I.
  • Journal Title: Biochem Biophys Res Commun. Volume: 482 Issue: 2 Pages: 375-381
  • DOI: 10.1016/j.bbrc.2016.11.070
  • Peer Reviewed
• [Journal Article] Role of chemokine C-C motif ligand-1 in acute and chronic pulmonary inflammations. (2016)
  • Author(s): Kishi H, Sato M, Shibata Y, Sato K, Inoue S, Abe S, Kimura T, Nishiwaki M, Yamauchi K, Nemoto T, Igarashi A, Tokairin Y, Nakajima O, Kubota I.
  • Journal Title: Springerplus Volume: 5 Issue: 1 Pages: 1241-1241
  • DOI: 10.1186/s40064-016-2904-z
  • Peer Reviewed / Open Access
• [Journal Article] Serum uric acid levels and mortality in the Japanese population: the Yamagata (Takahata) study. (2016)
  • Author(s): Kamei K, Konta T, Ichikawa K, Sato H, Suzuki N, Kabasawa A, Suzuki K, Hirayama A, Shibata Y, Watanabe T, Kato T, Ueno Y, Kayama T, Kubota I.
  • Journal Title: Clin Exp Nephrol. Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Reference values of MostGraph measures for middle-aged and elderly Japanese individuals who participated in annual health checkups. (2016)
  • Author(s): Abe Y, Shibata Y, Igarashi A, Inoue S, Sato K, Sato M, Nemoto T, Kobayashi M, Nishiwaki M, Kimura T, Tokairin Y, Kayama T, Kubota I
  • Journal Title: Ｒｅｓｐｉｒａｔｏｒｙ Ｉｎｖｅｓｔｉｇａｔｉｏｎ Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low arterial blood oxygenation is associated with calcification of the coronary arteries in patients with chronic obstructive pulmonary disease. (2015)
  • Author(s): Inoue S, Shibata Y, Kishi H, Hasegawa H, Nitobe J, Iwayama T, Yashiro Y, Nemoto T, Sato K, Nakano H, Sato M, Nunomiya K, Aida Y, Yamauchi K, Igarashi A, Abe S, Kubota I.
  • Journal Title: Respir Investig Volume: 53 Issue: 3 Pages: 111-116
  • DOI: 10.1016/j.resinv.2015.01.002
  • Peer Reviewed
• [Journal Article] SCGB3A2 Inhibits Acrolein-Induced Apoptosis through Decreased p53 Phosphorylation (2015)
  • Author(s): Kurotani R, Shima R, Miyano Y, Sakahara S, Matsumoto Y, Shibata Y, Abe, Kimura S.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 48 Issue: 2 Pages: 61-68
  • DOI: 10.1267/ahc.14065
  • NAID: 130005068296
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Slight increase in urinary albumin excretion within the normal range predicts incident hypertension in a community-based Japanese population: the Takahata study (2015)
  • Author(s): Hirayama A, Konta T, Hozawa A, Kawasaki R, Watanabe T, Shibata Y, Kayama T, Fukao A, Kubota I
  • Journal Title: Hypertension Research Volume: 38 Issue: 1 Pages: 56-60
  • DOI: 10.1038/hr.2014.117
  • Peer Reviewed / Open Access
• [Journal Article] Association of heart-type fatty acid-binding protein with cardiovascular risk factors and all-cause mortality in the general population: the Takahata study. (2014)
  • Author(s): Otaki Y, Watanabe T, Takahashi H, Hirayama A, Narumi T, Kadowaki S, Honda Y, Arimoto T, Shishido T, Miyamoto T, Konta T, Shibata Y, Fukao A, Daimon M, Ueno Y, Kato T, Kayama T, Kubota I.
  • Journal Title: PLoS One Volume: 9 Issue: 5 Pages: e94834-e94834
  • DOI: 10.1371/journal.pone.0094834
  • Peer Reviewed / Open Access
• [Journal Article] Association between plasma adiponectin levels and decline in forced expiratory volume in 1 s in a general Japanese population: the Takahata study. (2014)
  • Author(s): Sato K, Shibata Y, Abe S, Inoue S, Igarashi A, Yamauchi K, Aida Y, Nunomiya K, Nakano H, Sato M, Kimura T, Nemoto T, Watanabe T, Konta T, Ueno Y, Kato T, Kayama T, Kubota I.
  • Journal Title: Int J Med Sci. Volume: 11 Issue: 8 Pages: 758-764
  • DOI: 10.7150/ijms.8919
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of elastase-pulmonary emphysema in dominant-negative MafB transgenic mice. (2014)
  • Author(s): Aida Y, Shibata Y, Abe S, Inoue S, Kimura T, Igarashi A, Yamauchi K, Nunomiya K, Kishi H, Nemoto T, Sato M, Sato-Nishiwaki M, Nakano H, Sato K, Kubota I
  • Journal Title: Int J Biol Sci. Volume: 10 Issue: 8 Pages: 882-894
  • DOI: 10.7150/ijbs.8737
  • Peer Reviewed / Open Access
• [Presentation] 鉄欠乏状態下での喫煙曝露の影響についての検討 (2017)
  • Author(s): 佐藤建人等
  • Organizer: 第５７回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム（東京千代田区）
  • Year and Date: 2017-04-21
• [Presentation] Serum hepcidin and iron are associated with non-small cell lung cancer stage (2016)
  • Author(s): Sato K, Shibata Y, Inoue S, Igarash A, Tokairin Y, Yamauchi K, Kimura T, Nemoto T, Sato M, Nakano H, Abe Y, Kubota I
  • Organizer: ERS International Congress 2016
  • Place of Presentation: London (United Kingdom of Great Britain）
  • Year and Date: 2016-09-03
  • Int'l Joint Research
• [Presentation] Effect of Iron Deficiency on Lipopolysaccharide-Induced Acute Lung Injury. (2016)
  • Author(s): Sato K, Abe Y, Kobayashi M, Sato M, Nemoto T, Nishiwaki M, Kimura T, Yamauchi K, Igarashi A, Tokairin Y, Inoue S, Kubota I, Shibata Y
  • Organizer: American Thoracic Society International Conference 2016
  • Place of Presentation: San Francisco (America)
  • Year and Date: 2016-05-14
  • Int'l Joint Research
• [Presentation] マウス組織肺におけるIreb2の局在と発現の検討 (2016)
  • Author(s): 佐藤正道
  • Organizer: 第５６回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-04-08
• [Presentation] 高齢者肺炎における血清鉄と予後の関係 (2016)
  • Author(s): 佐藤建人
  • Organizer: 第５６回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-04-08
• [Presentation] 低鉄状態がLipopolysaccharide誘発急性肺障害に及ぼす影響についての検討 (2016)
  • Author(s): 佐藤建人
  • Organizer: 第５６回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-04-08
• [Presentation] 鉄欠乏状態における肺の鉄代謝の解析 (2015)
  • Author(s): 佐藤建人
  • Organizer: 第55回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
  • Year and Date: 2015-04-17 – 2015-04-19