Investigation of asbestos-induced carcinogenesis via actin-binding protein, p116Rip.

• Project/Area Number: 26461180
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Gunma University
• Principal Investigator: YASUHIKO KOGA 群馬大学, 大学院医学系研究科, 助教 (10533862)
• Co-Investigator(Renkei-kenkyūsha): KAIRA KYOUICHI 群馬大学, 医学部, 教授 (40400783) ; DOBASHI KUNIO 群馬大学, 保健学科, 教授 (00241894)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 非閉塞性肺疾患 / 肺線維症 / 呼吸器感染症 / アスベスト / p116Rip / アクチン / EMT / 上皮間葉移行 / 元素解析 / PIXE / アクチン結合タンパク / 肺癌 / ミオシン

Outline of Final Research Achievements

It has been reported that p116Rip has an ability to interact with cytoskeletal molecules such as myosin, actin, and myosin phosphatase, suggesting that p116Rip affects cell motility. However, the role of p116Rip has not been investigated well. In this study, we focused on the actin which binding to asbestos in vitro and on the role of p116Rip interacting with actin. We investigated the localization of p116Rip in lung tissues in patients with asbestos exposure and clarified the co-localization of p116Rip with EMT markers such as vimentin, β-catenin, and α-smooth muscle actin. The results suggested that p116Rip has an interaction with EMT markers and plays a role of asbestos-induced carcinogenesis.

Research Products

• [Journal Article] アスベスト関連肺腫瘍における吸入分子分布とp116Ripの発現解析について. (2017)
  • Author(s): 古賀 康彦
  • Journal Title: BIO CLINICA Volume: 6 Pages: 142-145
• [Journal Article] Isoniazid-induced pure red cell aplasia in a patient with sarcoidosis, a patient summary and review of the literature (2017)
  • Author(s): Yasuyuki Saito, Yuri Sawada, Yasuhiko Koga, Noriaki Sunaga, Yusuke Tsukagoshi, Yoshimasa Hachisu, Takashi Osaki, Reiko Sakurai, Kyoichi Kaira, Akihiro Ono, Ken Sato, Hiromi Koiso, Junko Hirato, Tetsunari Oyama, Takeshi Hisada, Masanobu Yamada
  • Journal Title: Internal Medicine Volume: 印刷中
  • NAID: 130006856740
  • Peer Reviewed / Open Access
• [Journal Article] Elemental and immunohistochemical analysis of the lungs and hilar lymph node in a patient with asbestos exposure, a pilot study. (2016)
  • Author(s): Koga Y, Satoh T, Kaira K, Koka M, Hisada T, Hirato J, Altan B, Yatomi M, Ono A, Kamide Y, Shimizu Y, Aoki-Saito H, Tsurumaki H, Shimizu K, Mogi A, Ishizuka T, Yamada M, Dobashi K.
  • Journal Title: Environ Health Prev Med. Volume: 6 Issue: 6 Pages: 492-500
  • DOI: 10.1007/s12199-016-0576-5
  • NAID: 50010159091
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CREB regulates TNF-α-induced GM-CSF secretion via p38 MAPK in human lung fibroblasts (2016)
  • Author(s): Yasuhiko Koga, Takeshi Hisada, Tamotsu Ishizuka, Mitsuyoshi Utsugi,, Akihiro Ono, Masakiyo Yatomi, Yosuke Kamide, Haruka Aoki-Saito, Hiroaki Tsurumaki, Kunio Dobashi, Masanobu Yamada
  • Journal Title: Allergology International Volume: 印刷中
  • NAID: 130005431338
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phase I dose escalation study of amrubicin plus paclitaxel in previously treated advanced non-small cell lung cancer. (2016)
  • Author(s): Kaira, Kyoichi; Sunaga, Noriaki; Imai, Hisao; Kamide, Yosuke; Koga, Yasuhiko; Ono, Akihiro; Kuwako, Tomohito; Masuda, Tomomi; Hisada, Takeshi; Ishizuka, Tamotsu; Yamada, Masanobu.
  • Journal Title: International Journal of Clinical Oncology Volume: 21(2) Issue: 2 Pages: 240-7
  • DOI: 10.1007/s10147-015-0883-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reversible Hypopituitarism Associated with Intravascular Large B-Cell Lymphoma: Case Report of Successful Immunochemotherapy (2016)
  • Author(s): Sawada Y, Ishii S, Koga Y, Tomizawa T, Matsui A, Tomaru T, Ozawa A, Shibusawa N, Satoh T, Shimizu H, Hirato J, Yamada M.
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 238 Issue: 3 Pages: 197-203
  • DOI: 10.1620/tjem.238.197
  • NAID: 130005131608
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 17(R)-resolvin D1 ameliorates bleomycin-induced pulmonary fibrosis in mice. (2015)
  • Author(s): Yatomi M, Hisada T, Ishizuka T, Koga Y, Ono A, Kamide Y, Seki K, Aoki-Saito H, Tsurumaki H, Sunaga N, Kaira K, Dobashi K, Yamada M, Okajima F.
  • Journal Title: PHYSIOLOGICAL REPORTS Volume: 3 Issue: 12 Pages: 12628-12628
  • DOI: 10.14814/phy2.12628
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Acidic environment augments FcεRI-mediated production of IL-6 and IL-13 in mast cells. (2015)
  • Author(s): Y. Kamide, T. Ishizuka, M. Tobo, H. Tsurumaki, H. Aoki, C. Mogi, T. Nakakura, M. Yatomi, A. Ono, Y. Koga, K. Sato, T. Hisada, K. Dobashi, M. Yamada, F. Okajima
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 464 Issue: 3 Pages: 949-955
  • DOI: 10.1016/j.bbrc.2015.07.077
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hydroxyurea-induced Pneumonitis in a Patient with Chronic Myelomonocytic Leukemia: An Autopsy Case (2015)
  • Author(s): Imai H, Matsumura N, Yamazaki Y, Kanayama Y, Masuda T, Kuwako T, Kamide Y, Tomizawa T, Matsumoto S, Mitsui T, Kaira K, Ono A, Koga Y, Shibusawa N, Sunaga N, Hisada T, Yokoo H, Yamada M.
  • Journal Title: Internal Medicine Volume: 54 Issue: 24 Pages: 3171-3176
  • DOI: 10.2169/internalmedicine.54.5069
  • NAID: 130005114246
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Anaplastic Lymphoma Kinase (ALK) Rearrangement-positive Lung Cancer with Transformation to Pleomorphic Carcinoma (2015)
  • Author(s): Kamide Y, Kaira K, Watanabe T, Kuribayashi S, Ozawa A, Koga Y, Ono A, Sunaga N, Hisada T, Oyama T, Yamada M.
  • Journal Title: Internal Medicine Volume: 54 Issue: 22 Pages: 2905-2909
  • DOI: 10.2169/internalmedicine.54.4987
  • NAID: 130005108841
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pulmonary Aluminosis Diagnosed with In-air Microparticle Induced X-ray Emission Analysis of Particles (2015)
  • Author(s): Chino, Haruka; Hagiwara, Eri; Sugisaki, Midori; Baba, Tomonori; Koga, Yasuhiko; Hisada, Takeshi; Kaira, Kyoichi; Okudela, Koji; Takemura, Tamiko; Dobashi, Kunio; Ogura, Takashi.
  • Journal Title: Internal Medicine Volume: 54 Issue: 16 Pages: 2035-2040
  • DOI: 10.2169/internalmedicine.54.4246
  • NAID: 130005092917
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Primary Sjogren’s syndrome presenting unilateral bloody pleural effusion complicated with Waldenstrom’s macrogloburinemia (2017)
  • Author(s): Yasuhiko Koga, Kazuhiko Uchiyama, Norifumi Tsukamoto, Nobuyuki Shibusawa, Aska Jingu, Mitsuyoshi Utsugi, Satoru Kakizaki, Junko Hirato, Kunio Dobashi, Takeshi Hisada, Masanobu Yamada
  • Organizer: Conference of International Union Against Tuberculosis and Lung Disease Asia Pacific Region
  • Place of Presentation: Japan, Tokyo
  • Int'l Joint Research
• [Presentation] アスベスト暴露患者の肺及び肺門リンパ節を用いた元素解析と免疫組織学的検討 (2017)
  • Author(s): 古賀康彦,解良恭一,BolagAltan,久田剛志,土橋邦生,佐藤隆博,山田尚人
  • Organizer: QST高崎研シンポジウム
  • Place of Presentation: 群馬、高崎
• [Presentation] CREB regulates TNF-α-induced GM-CSF secretion via p38 MAPK in human lung fibroblasts (2016)
  • Author(s): Y.Koga,T.Hisada,T.Ishizuka,M.Utsugi,A.Ono,M.Yatomi,Y.Kamide,H.SaitoAoki,H.Tsurumaki,Y.Hachisu,K.Dobashi,M.Yamada
  • Organizer: Intel ASMA
  • Place of Presentation: Japan, Fukuoka
  • Int'l Joint Research
• [Presentation] Study of the patients under treatment of respiratory disorders that were complicated with mediastinal emphysema. (2016)
  • Author(s): A.Ono,K.Dobashi,Y.Koga,Y.Hachisu,H.Tsurumaki,M.Yatomi,N.Kasahara,Y.Tsukagosi,N.Sunaga,T.Maeno,T.Hisada T.
  • Organizer: Asian Pacific Society of Respilrology
  • Place of Presentation: Thailand, Bangkok
  • Int'l Joint Research
• [Presentation] Inhaled Inorganic Elemental Analysis of Idiopathic Pulmonary Fibrosis by In-air Microparticle Induced X-ray Emission Analysis. (2016)
  • Author(s): Y.Koga,T.Satoh,K.Kaira,M.Koka,Y.Hachisu,T.Hisada,A.Ono,Y.Shimizu,T.Maeno,K.Shimizu,A.Mogi,T.Ishizuka,M.Yamada, K.Dobashi.
  • Organizer: American Thoracic Society Conference
  • Place of Presentation: U.S.A. San, Francisco
  • Int'l Joint Research
• [Presentation] Elemental and immunohistochemical analysis of the lung and hilar lymph node in a patient with asbestos exposure (2016)
  • Author(s): Y. Koga, T. Satoh, K. Kaira, M. Koka, T. Hisada, M. Yatomi, A. Ono, Y. Kamide, Y. Shimizu, H. Aoki-Saito, H. Tsurumaki, T. Ishizuka, M. Yamada, K. Dobashi
  • Organizer: Japanese Respiratory Society
  • Place of Presentation: 京都、京都国際会館
• [Presentation] The opposite effect of p38 MAP kinase and cAMP-elevating agents, Roflumilast and Rolipram, in the GM-CSF secretion from human lung fibroblasts (2016)
  • Author(s): Yasuhiko Koga, Takeshi Hisada, Tamotsu Ishizuka, Mitsuyoshi Utsugi, Akihiro Ono, Masakiyo Yatomi, Yosuke Kamide, Haruka Aoki-Saito, Hiroaki Tsurumaki, Yoshimasa Hachisu, Yasuyuki Saito, Toshitaka Maeno, Kunio Dobashi, Masanobu Yamada
  • Organizer: Japanese Society of Allergology
  • Place of Presentation: Japan, Tokyo
• [Presentation] 特発性肺線維症患者における予後予測因子としての肺内吸入元素の検討 (2016)
  • Author(s): 古賀 康彦, 佐藤 隆博, 解良 恭一, 江夏 昌志, 蜂巣 克昌, 久田 剛志, 矢冨 正清, 小野 昭浩, 清水 泰生, 土橋 邦生
  • Organizer: 日本職業・環境アレルギー学会
  • Place of Presentation: 大阪
• [Presentation] アスベスト暴露患者の肺内リンパ節を用いた大気マイクロPIXEの元素解析と免疫組織学的検討 (2015)
  • Author(s): 古賀 康彦, 佐藤 隆博, 解良 恭一, 江夏 昌志, 久田 剛志,矢冨 正清,小野 昭浩, 上出 庸介, 清水 泰生, 青木 悠,鶴巻 寛朗,石塚 全, 山田 正信,土橋 邦生
  • Organizer: 第31回PIXEシンポジウム
  • Place of Presentation: 高崎、 国立研究開発法人日本原子力研究開発機構 高崎量子応用研究所
  • Year and Date: 2015-10-28
• [Presentation] 大気マイクロPIXEによるアスベスト暴露患者の肺内リンパ節の元素解析と免疫組織学的検討 (2015)
  • Author(s): 古賀 康彦, 佐藤 隆博, 解良 恭一, 江夏 昌志, 久田 剛志,矢冨 正清,小野 昭浩, 上出 庸介, 清水 泰生, 青木 悠,鶴巻 寛朗,石塚 全, 山田 正信,土橋 邦生
  • Organizer: 第１０回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎、 国立研究開発法人日本原子力研究開発機構 高崎量子応用研究所
  • Year and Date: 2015-10-09
• [Presentation] Cyclic AMP signaling prevents TNFα-induced GM CSF secretion in human lung fibroblast (2015)
  • Author(s): 古賀 康彦、 矢冨正清、上出庸介、関香織、青木悠、鶴巻寛朗、小野昭浩、齋藤康之、蜂巣克昌、久田剛志、石塚全、土橋邦生、山田正信
  • Organizer: 第25回国際喘息学会日本・北アジア部会
  • Place of Presentation: 横浜、横浜ニューグランドホテル
  • Year and Date: 2015-09-03
• [Presentation] 大気マイクロPIXEによるアスベスト暴露患者の肺内リンパ節の元素解析 (2015)
  • Author(s): 古賀 康彦,土橋 邦生,解良 恭一, 佐藤 隆博, 久田 剛志, 矢冨 正清,小野 昭浩,青木 悠,鶴巻 寛朗,石塚 全,山田 正信
  • Organizer: 第46回日本職業・環境アレルギー学会
  • Place of Presentation: 東京、東京大学伊藤国際学術研究センター
  • Year and Date: 2015-07-03
• [Presentation] p116Rip proteinの気道培養細胞でのGM-CSF分泌に及ぼす影響について (2015)
  • Author(s): 古賀 康彦、 矢富正清、上出庸介、関香織、青木悠、鶴巻寛朗、小野昭浩、今井久雄、砂長則明、久田剛志、石塚全、土橋邦生、山田正信
  • Organizer: 第64回日本アレルギー学会
  • Place of Presentation: 東京、グランドプリンスホテル 新高輪
  • Year and Date: 2015-05-27
• [Presentation] In-air microparticle induced X-ray emission analysis of inhaled particulates in thoracic lymph node in a patient with asbestos exposure (2015)
  • Author(s): Y. Koga, K. Dobashi, K. Kaira, T. Satoh, M. Koka, N. Yamada, T. Hisada, M. Yatomi, A. Ono, Y. Kamide, K. Seki, H. Aoki, H. Tsurumaki, M. Yamada.
  • Organizer: American Thoracic Society Conference
  • Place of Presentation: Denver, U.S.A.
  • Year and Date: 2015-05-18
  • Int'l Joint Research
• [Presentation] p116Rip proteinの肺線維芽細胞HFL-1の遊走能に及ぼす影響について (2015)
  • Author(s): 古賀 康彦、 矢冨正清、上出庸介、関香織、青木悠、鶴巻寛朗、解良恭一、小野昭浩、砂長則明、久田剛志、桑子智人、増田友美、石塚全、土橋邦生、山田正信
  • Organizer: 第55回 日本呼吸器学会
  • Place of Presentation: 東京、東京国際フォーラム
  • Year and Date: 2015-04-25
• [Presentation] The efficacy of in-air microanalysis of particles in interstitial pneumonia lung tissue (2014)
  • Author(s): 古賀 康彦
  • Organizer: 第９回高崎量子応用研究シンポジウム
  • Place of Presentation: 群馬 高崎シティギャラリー
  • Year and Date: 2014-10-10
• [Presentation] p116Rip elimination enhances PDGF-induced cell migration (2014)
  • Author(s): 古賀 康彦
  • Organizer: 第24回国際喘息学会日本・北アジア部会
  • Place of Presentation: 名古屋 ウインクあいち
  • Year and Date: 2014-07-19
• [Presentation] 間質性肺炎症例におけるパラフィン切片を用いたIn-air micro PIXE解析による肺内微粒子の元素解析 (2014)
  • Author(s): 古賀 康彦
  • Organizer: 日本職業・環境アレルギー学会
  • Place of Presentation: 福岡 福岡ソフトリサーチパーク(SRP)センタービル
  • Year and Date: 2014-06-26
• [Presentation] The efficacy of in-air microanalysis of particles in interstitial pneumonia lung tissue in patients with or without occupational exposure. (2014)
  • Author(s): YASUHIKO KOGA
  • Organizer: American thoracic society
  • Place of Presentation: San Diego, U.S.A.
  • Year and Date: 2014-05-19
• [Presentation] p116Rip proteinの気道培養細胞での炎症性サイトカイン分泌に及ぼす影響について (2014)
  • Author(s): 古賀 康彦
  • Organizer: 第26回日本アレルギー学会
  • Place of Presentation: 京都 国立京都国際会館
  • Year and Date: 2014-05-10
• [Presentation] p116Rip proteinの気道培養細胞でのサイトカイン分泌に及ぼす影響について (2014)
  • Author(s): 古賀 康彦
  • Organizer: 第54回 日本呼吸器学会
  • Place of Presentation: 大阪 大阪国際会議場
  • Year and Date: 2014-04-25
• [Book] 職業性アレルギー疾患診療ガイドライン2016 (2016)
  • Author(s): 土橋邦生、古賀康彦ら
  • Total Pages: 172
  • Publisher: 職業性アレルギー疾患診療ガイドライン2016