• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Reprograming of lung epithelial cells

Research Project

Project/Area Number 26461185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

SAITO Akira  東京大学, 保健・健康推進本部, 助教 (90591412)

Research Collaborator NAGASE Takahide  
OHSHIMA Mitsuhiro  
YAMAGUCHI Yoko  
SUZUKI Hiroshi  
HORIE Masafumi  
NOGUCHI Satoshi  
OKUNISHI Katsuhide  
ONO Naoaki  
MICKE Patrick  
KACZKOWSKI Bogumil  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsリプログラミング / 遺伝子導入 / 肺上皮細胞 / 肺線維芽細胞 / 小細胞肺癌 / 非小細胞肺癌 / 肺線維症 / トランスクリプトーム / 転写因子 / 肺胞上皮細胞 / トランスクリプト―ム / 転写ネットワーク / 三次元培養 / 動物モデル
Outline of Final Research Achievements

We explored optimal settings for gene transduction by electroporation in human iPS cells and dermal fibroblasts, and determined the condition for low cytotoxicity and high transduction efficiency. We constructed expression vectors for TTF-1, YAP/TAZ, and FOXA2, and attempted to induce cellular differentiation via ectopic expression of these factors. We also tested the effects of TGF-beta/BMP-4/FGF-2 ligands/inhibitors, demethylating agent, and HDAC inhibitor. In an attempt to establish suitable conditions for lung epithelial cell differentiation, serum-free media, collagen gels embedded with lung fibroblasts, three-dimensional and air-liquid interface cultures were utilized. Elevated expression of lung epithelial cell markers (SPC, CC10, and FoxJ1) were confirmed by quantitative PCR, suggesting that lung epithelial cell differentiation with a certain level was achieved through combinations of gene transfer and culture methods.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (14 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 14 results,  Open Access: 6 results,  Acknowledgement Compliant: 7 results) Presentation (4 results)

  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] TAZ contributes to pulmonary fibrosis by activating profibrotic functions of lung fibroblasts.2017

    • Author(s)
      Noguchi S, Saito A, Mikami Y, Urushiyama H, Horie M, Matsuzaki H, Takeshima H, Makita K, Miyashita N, Mitani A, Jo T, Yamauchi Y, Terasaki Y, Nagase T.
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 42595-42595

    • DOI

      10.1038/srep42595

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Regulation of TGF-β-mediated endothelial-mesenchymal transition by microRNA-272017

    • Author(s)
      Suzuki HI, Katsura A, Mihira H, Horie M, Saito A, Miyazono K.
    • Journal Title

      J Biochem.

      Volume: -- Issue: 5 Pages: 417-420

    • DOI

      10.1093/jb/mvx017

    • NAID

      40021219791

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Integrated pathway-based transcription regulation network mining and visualization based on gene expression profiles.2016

    • Author(s)
      Kibinge N, Ono N, Horie M, Sato T, Sugiura T, Altaf-Ul-Amin M, Saito A, Kanaya S
    • Journal Title

      J Biomed Inform

      Volume: 61 Pages: 194-202

    • DOI

      10.1016/j.jbi.2016.04.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] YAP and TAZ modulate cell phenotype in a subset of small cell lung cancer.2016

    • Author(s)
      Horie M, Saito A, Ohshima M, Suzuki HI, Nagase T.
    • Journal Title

      Cancer Sci

      Volume: 107 Issue: 12 Pages: 1755-1766

    • DOI

      10.1111/cas.13078

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Transcriptome analysis of periodontitis-associated fibroblasts by CAGE sequencing identified DLX5 and RUNX2 long variant as novel regulators involved in periodontitis.2016

    • Author(s)
      Horie M, Yamaguchi Y, Saito A, Nagase T, Lizio M, Itoh M, Kawaji H, Lassmann T, Carninci P, Forrest AR, Hayashizaki Y, Suzutani T, Kappert K, Micke P, Ohshima M.
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1

    • DOI

      10.1038/srep33666

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Profiling cancer testis antigens in non-small-cell lung cancer.2016

    • Author(s)
      Djureinovic D, Hallstrom BM, Horie M, Mattsson JS, La Fleur L, Fagerberg L, Brunnstrom H, Lindskog C, Madjar K, Rahnenfuhrer J, Ekman S, Stahle E, Koyi H, Branden E, Edlund K, Hengstler JG, Lambe M, Saito A, Botling J, Ponten F, Uhlen M, Micke P
    • Journal Title

      JCI Insight

      Volume: 1(10) Issue: 10

    • DOI

      10.1172/jci.insight.86837

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fibroblast VEGF-receptor 1 expression as molecular target in periodontitis2016

    • Author(s)
      Ohshima M, Yamaguchi Y, Ambe K, Horie M, Saito A, Nagase T, Nakashima K, Ohki H, Kawai T, Abiko Y, Micke P, Kappert K
    • Journal Title

      J Clin Periodontol

      Volume: 43(2) Issue: 2 Pages: 128-37

    • DOI

      10.1111/jcpe.12495

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hippo and TGF-β interplay in the lung field2015

    • Author(s)
      Saito A, Nagase T
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol

      Volume: 309(8) Issue: 8 Pages: L756-L767

    • DOI

      10.1152/ajplung.00238.2015

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional Co-culture Model for Tumor-stromal Interaction2015

    • Author(s)
      Horie M, Saito A, Yamaguchi Y, Ohshima M, Nagase T
    • Journal Title

      Journal of Visualized Experiments

      Volume: 96 Issue: 96

    • DOI

      10.3791/52469

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Smad3 and TTF-1/NKX2-1 complex regulates Smad4-independent gene expression2014

    • Author(s)
      Isogaya K, Koinuma D, Tsutsumi S, Saito RA, Miyazawa K, Aburatani H, Miyazono K.
    • Journal Title

      Cell Research

      Volume: 24 Issue: 8 Pages: 994-1008

    • DOI

      10.1038/cr.2014.97

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histamine induces human lung fibroblast-mediated collagen gel contraction via histamine H1 receptor.2014

    • Author(s)
      5.Horie M, Saito A, Yamauchi Y, Mikami Y, Sakamoto M, Jo T, Nakajima J, Takizawa H, Nagase T, Kohyama T.
    • Journal Title

      Exp Lung Res.

      Volume: 40 Issue: 5 Pages: 222-36

    • DOI

      10.3109/01902148.2014.900155

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] An integrative analysis of the tumorigenic role of TAZ in human non-small cell lung cancer.2014

    • Author(s)
      3.Noguchi S, Saito A, Horie M, Mikami Y, Suzuki HI, Morishita Y, Ohshima M, Abiko Y, Mattsson JS, König H, Lohr M, Edlund K, Botling J, Micke P, Nagase T
    • Journal Title

      Clin Cancer Res.

      Volume: 20 Issue: 17 Pages: 4660-72

    • DOI

      10.1158/1078-0432.ccr-13-3328

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Novel swine model of transfusion-related acute lung injury.2014

    • Author(s)
      Okazaki H, Ishikawa O, Iijima T, Kohira T, Teranishi M, Kawasaki S, Saito A, Mikami Y, Sugiura A, Hashimoto S, Shimada E, Uchikawa M, Matsuhashi M, Tsuno NH, Tanaka M, Kiyokawa N, Fujimoto J, Nagase T, Tadokoro K, Takahashi K.
    • Journal Title

      Transfusion.

      Volume: 54 Issue: 12 Pages: 3097-3107

    • DOI

      10.1111/trf.12766

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differential knockdown of TGF-β ligands in a three-dimensional co-culture tumor- stromal interaction model of lung cancer.2014

    • Author(s)
      6.Horie M, Saito A, Noguchi S, Yamaguchi Y, Ohshima M, Morishita Y, Suzuki HI, Kohyama T, Nagase T.
    • Journal Title

      BMC Cancer.

      Volume: 14 Issue: 1 Pages: 580-580

    • DOI

      10.1186/1471-2407-14-580

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 肺小細胞癌のトランスクリプトーム解析2016

    • Author(s)
      堀江 真史、齋藤  朗、長瀬 隆英
    • Organizer
      日本癌学会
    • Place of Presentation
      横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 肺小細胞癌のトランスクリプトーム解析2016

    • Author(s)
      堀江 真史、齋藤  朗、長瀬 隆英
    • Organizer
      日本呼吸器学会
    • Place of Presentation
      京都
    • Year and Date
      2016-04-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Hippo pathwayの構成因子であるTAZの肺線維症における役割2016

    • Author(s)
      野口 智史、齋藤  朗、三上  優、漆山 博和、寺崎 泰弘、堀江 真史、松崎 博崇、竹島 英之、槇田 広佑、三谷 明久、山内 康宏、長瀬 隆英
    • Organizer
      日本呼吸器学会
    • Place of Presentation
      京都
    • Year and Date
      2016-04-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] エピソーマルベクターを用いた肺上皮細胞へのダイレクトリプログラミングの試み2014

    • Author(s)
      堀江 真史,齋藤  朗,野口 智史,長瀬 隆英
    • Organizer
      第35回日本炎症・再生医学会
    • Place of Presentation
      沖縄
    • Year and Date
      2014-07-01 – 2014-07-04
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi