Reprograming of lung epithelial cells
Project/Area Number |
26461185
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Respiratory organ internal medicine
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Research Institution | The University of Tokyo |
Principal Investigator |
SAITO Akira 東京大学, 保健・健康推進本部, 助教 (90591412)
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Research Collaborator |
NAGASE Takahide
OHSHIMA Mitsuhiro
YAMAGUCHI Yoko
SUZUKI Hiroshi
HORIE Masafumi
NOGUCHI Satoshi
OKUNISHI Katsuhide
ONO Naoaki
MICKE Patrick
KACZKOWSKI Bogumil
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | リプログラミング / 遺伝子導入 / 肺上皮細胞 / 肺線維芽細胞 / 小細胞肺癌 / 非小細胞肺癌 / 肺線維症 / トランスクリプトーム / 転写因子 / 肺胞上皮細胞 / トランスクリプト―ム / 転写ネットワーク / 三次元培養 / 動物モデル |
Outline of Final Research Achievements |
We explored optimal settings for gene transduction by electroporation in human iPS cells and dermal fibroblasts, and determined the condition for low cytotoxicity and high transduction efficiency. We constructed expression vectors for TTF-1, YAP/TAZ, and FOXA2, and attempted to induce cellular differentiation via ectopic expression of these factors. We also tested the effects of TGF-beta/BMP-4/FGF-2 ligands/inhibitors, demethylating agent, and HDAC inhibitor. In an attempt to establish suitable conditions for lung epithelial cell differentiation, serum-free media, collagen gels embedded with lung fibroblasts, three-dimensional and air-liquid interface cultures were utilized. Elevated expression of lung epithelial cell markers (SPC, CC10, and FoxJ1) were confirmed by quantitative PCR, suggesting that lung epithelial cell differentiation with a certain level was achieved through combinations of gene transfer and culture methods.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] TAZ contributes to pulmonary fibrosis by activating profibrotic functions of lung fibroblasts.2017
Author(s)
Noguchi S, Saito A, Mikami Y, Urushiyama H, Horie M, Matsuzaki H, Takeshima H, Makita K, Miyashita N, Mitani A, Jo T, Yamauchi Y, Terasaki Y, Nagase T.
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Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 42595-42595
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Transcriptome analysis of periodontitis-associated fibroblasts by CAGE sequencing identified DLX5 and RUNX2 long variant as novel regulators involved in periodontitis.2016
Author(s)
Horie M, Yamaguchi Y, Saito A, Nagase T, Lizio M, Itoh M, Kawaji H, Lassmann T, Carninci P, Forrest AR, Hayashizaki Y, Suzutani T, Kappert K, Micke P, Ohshima M.
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Journal Title
Sci Rep
Volume: 6
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Profiling cancer testis antigens in non-small-cell lung cancer.2016
Author(s)
Djureinovic D, Hallstrom BM, Horie M, Mattsson JS, La Fleur L, Fagerberg L, Brunnstrom H, Lindskog C, Madjar K, Rahnenfuhrer J, Ekman S, Stahle E, Koyi H, Branden E, Edlund K, Hengstler JG, Lambe M, Saito A, Botling J, Ponten F, Uhlen M, Micke P
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Journal Title
JCI Insight
Volume: 1(10)
Issue: 10
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Fibroblast VEGF-receptor 1 expression as molecular target in periodontitis2016
Author(s)
Ohshima M, Yamaguchi Y, Ambe K, Horie M, Saito A, Nagase T, Nakashima K, Ohki H, Kawai T, Abiko Y, Micke P, Kappert K
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Journal Title
J Clin Periodontol
Volume: 43(2)
Issue: 2
Pages: 128-37
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] An integrative analysis of the tumorigenic role of TAZ in human non-small cell lung cancer.2014
Author(s)
3.Noguchi S, Saito A, Horie M, Mikami Y, Suzuki HI, Morishita Y, Ohshima M, Abiko Y, Mattsson JS, König H, Lohr M, Edlund K, Botling J, Micke P, Nagase T
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Journal Title
Clin Cancer Res.
Volume: 20
Issue: 17
Pages: 4660-72
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Novel swine model of transfusion-related acute lung injury.2014
Author(s)
Okazaki H, Ishikawa O, Iijima T, Kohira T, Teranishi M, Kawasaki S, Saito A, Mikami Y, Sugiura A, Hashimoto S, Shimada E, Uchikawa M, Matsuhashi M, Tsuno NH, Tanaka M, Kiyokawa N, Fujimoto J, Nagase T, Tadokoro K, Takahashi K.
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Journal Title
Transfusion.
Volume: 54
Issue: 12
Pages: 3097-3107
DOI
Related Report
Peer Reviewed
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