The cross-talk between innate immunity and acquired immunity in exacerbation of airway inflammation in asthmatic attack
Project/Area Number |
26461186
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Respiratory organ internal medicine
|
Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
須田 隆文 浜松医科大学, 医学部, 教授 (30291397)
|
Research Collaborator |
MORI KAZUTAKA 浜松医科大学, 医学部, 大学院生
YAMANAKA KATSUMASA 浜松医科大学, 医学部, 大学院生
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 気管支喘息 / 気道炎症 / IL-17A / Toll like receptor / 炎症性サイトカイン / 自然免疫 / 獲得免疫 / 気道上皮細胞 / IL-17 / TLR3 / Toll-like receptor / polyI:C |
Outline of Final Research Achievements |
The interaction between IL-17A and TLR3 activation in airway epithelium remains poorly understood. In this study, we demonstrated that IL-17A and polyI:C, the ligand of TLR3, synergistically induced proinflammatory cytokine and chemokine (G-CSF, IL-8) mRNA and protein expression. Inhibition of the NF-κB pathway using NF-κB p65 siRNA, or BAY11-7082 (IκB-α inhibitor) attenuated the IL-17A/polyI:C-induced synergistic expression of proinflammatory cytokines. Knockdown of IRF3 using siRNA also decreased the synergistic gene expression. In western blotting analysis, co-treatment with IL-17A and polyI:C augmented IκB-α phosphorylation compared to polyI:C treatment alone. These findings demonstrate that IL-17A and TLR3 activation act in concert to potentiate proinflammatory responses in the airway epithelium via NF-κB/IRF3 activation, and that enhanced activation of the NF-κB pathway play a key role in the synergism.
|
Report
(4 results)
Research Products
(20 results)
-
[Journal Article] Efficacy of short-term prednisolone treatment in patients with chronic eosinophilic pneumonia2015
Author(s)
Oyama Y, Fujisawa T, Hashimoto D, Enomoto N, Nakamura Y, Inui N, Kuroishi S, Yokomura K, Toyoshima M, Yamada T, Shirai T, Masuda M, Yasuda K, Hayakawa H, Chida K, Suda T
-
Journal Title
Eur Respir J
Volume: 45
Issue: 6
Pages: 1624-1631
DOI
Related Report
Peer Reviewed
-
[Journal Article] Prognostic Significance of Anti-Aminoacyl-tRNA Synthetase Antibodies in Polymyositis/Dermatomyositis-Associated Interstitial Lung Disease: A Retrospective Case Control Study.2015
Author(s)
Hozumi H, Enomoto N, Kono M, Fujisawa T, Inui N, Nakamura Y, Sumikawa H, Johkoh T, Nakashima R, Imura Y, Mimori T, Suda T
-
Journal Title
PLoS One
Volume: 19;10(3)
Issue: 3
Pages: e0120313-e0120313
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Treatment of acute exacerbation of idiopathic pulmonary fibrosis with direct hemoperfusion using a polymyxin B-immobilized fiber column improves survival2015
Author(s)
Enomoto N, Mikamo M, Oyama Y, Kono M, Hashimoto D, Fujisawa T, Inui N, Nakamura Y, Yasuda H, Kato A, Mimuro S, Doi M, Sato S, Suda T.
-
Journal Title
BMC Pulm Med
Volume: 15
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
[Journal Article] Evaluation of palonosetron and dexamethasone with or without aprepitant to prevent carboplatin-induced nausea and vomiting in patients with advanced non-small-cell lung cancer.2015
Author(s)
Kusagaya H, Inui N, Karayama M, Fujisawa T, Enomoto N, Kuroishi S, Nakamura Y, Matsuda H, Yokomura K, Koshimizu N, Toyoshima M, Imokawa S, Yamada T, Shirai T, Hayakawa H, Suda T.
-
Journal Title
Lung Cancer
Volume: 90
Issue: 3
Pages: 410-6
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
[Journal Article] Plasma connective tissue growth factor levels as potential biomarkers of airway obstruction in patients with asthma. Ann Allergy Asthma Immunol.2014
Author(s)
Kato M, Fujisawa T, Hashimoto D, Kono M, Enomoto N, Nakamura Y, Inui N, Hamada E, Miyazaki O, Kurashita S, Maekawa M, Suda T
-
Journal Title
Ann Allergy Asthma Immunol.
Volume: 113 (3)
Issue: 3
Pages: 295-300
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Quantitative analysis of lung elastic fibers in idiopathic pleuroparenchymal fibroelastosis (IPPFE): comparison of clinical, radiological, and pathological findings with those of idiopathic pulmonary fibrosis (IPF)2014
Author(s)
Enomoto N, Kusagaya H, Oyama Y, Kono M, Kaida Y, Kuroishi S, Hashimoto D, Fujisawa T, Yokomura K, Inui N, Nakamura Y, Suda T
-
Journal Title
BMC Pulm Med
Volume: 28
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
[Presentation] Clinical features and prognostic factors of myositis-associated interstitial lung diseases.2014
Author(s)
Fujisawa T, Hozumi H , Kono M, Enomoto N, Hashimoto D,NakamuraY, Inui N , YokomuraK, Koshimizu N, Toyoshima M,Shirai T, Yasuda K, Hayakawa H, Suda T.
Organizer
American Thoracic Society
Place of Presentation
San Diego
Year and Date
2014-05-18 – 2014-05-21
Related Report
-