Elucidation of the role of IL-23 in bronchial asthma caused by skin barrier dysfunction and drug discovery application

• Project/Area Number: 26461198
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Keio University
• Principal Investigator: Kagawa Shizuko 慶應義塾大学, 医学部(信濃町), 研究員 (80645507)
• Co-Investigator(Renkei-kenkyūsha): Fukunaga Koichi 慶應義塾大学, 医学部, 専任講師 (60327517) ; Suzuki Yusuke 慶應義塾大学, 医学部, 特任講師 (80306696) ; Kubo Akiharu 慶應義塾大学, 医学部, 専任講師 (70335256)
• Research Collaborator: Kabata Hiroki ; Masaki Katsunori ; Mochimaru Takao ; Matsusaka Masako
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 経皮感作 / 皮膚バリア / IL-23 / 気管支喘息 / 気道過敏性 / 好酸球 / 動物モデル / Th17

Outline of Final Research Achievements

Th17 cells are the main cells that produce IL-17A.We found that IL-23 is required for the differentiation of Th17 cells via a patch application on the skin.In oder to find a therapeutic target of percutaneous sensitized asthma, I focused on the role of IL-23 produced in the local skin region and the role of Th17 in cytokine enhancement by administrating anti-IL-23 or control IgG to a percutaneously sensitized asthma mouse model, and then evaluating it for eosinophilic airway inflammation In the group to which the anti-IL-23 antibody was administered during the sensitization phase, the production of OVA-specific IgG1 antibody tended to decrease compared to the group to which the control IgG antibody was administered, and furthermore, the bronchoalveolar lavage fluid eosinophil count and eosinophil infiltration in tissues were found to decrease significantly.

Research Products

• [Journal Article] Dual Role of Interleukin-23 in Epicutaneously-Sensitized Asthma in Mice (2014)
  • Author(s): Masaki K, Suzuki Y, Kagawa S, Kodama M, Kabata H, Miyata J, Tanaka K, Fukunaga K, Sayama K, Oguma T, Kimura T, Amagai M, Betsuyaku T, Asano K.
  • Journal Title: Allergology International Volume: 63 Issue: Supplement.1 Pages: S13-S22
  • DOI: 10.2332/allergolint.13-OA-0632
  • NAID: 130004477229
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Presentation] 経皮感作喘息モデルにおけるIL-23の役割 (2014)
  • Author(s): 正木克宜、加川志津子、別役智子、浅野浩一郎 ら
  • Organizer: 第26回日本アレルギー学会春季臨床学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-05-09 – 2014-05-11