MicroRNA and exosome in interstitial pneumonia

• Project/Area Number: 26461204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Nippon Medical School
• Principal Investigator: Kunugi Shinobu 日本医科大学, 医学部, 講師 (30350036)
• Co-Investigator(Kenkyū-buntansha): 寺崎 泰弘 日本医科大学, 医学部, 准教授 (50332870)
• Co-Investigator(Renkei-kenkyūsha): MIYAKE KOUICHI 日本医科大学, 医学部, 准教授 (90267211) ; NAGASAKA SHINYA 日本医科大学, 医学部, 助教 (00573239) ; TERASAKI MIKA 日本医科大学, 医学部, 助教 (50372785) ; SEIKE MASAHIRO 日本医科大学, 医学部, 准教授 (30366687) ; ISHIKAWA ARIMI 日本医科大学, 医学部, 研究員 (40598992) ; KUWAHARA NAOMI 日本医科大学, 医学部, 研究員 (00599011)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 気管支肺胞洗浄液 / マイクロRNA / エクソソーム / II型肺胞上皮 / 間質性肺炎 / miRNA / エクソゾーム / microRNA

Outline of Final Research Achievements

We performed micro RNA analysis of serum, bronchoalveolar lavage, and lung tissue of bleomycin-induced pneumonitis in mice. Particularly, We analyzed exosomal franction and others fraction of bronchoalveolar lavage.In fibrotic stage,the elevation of miR-150 were confirmed.

Research Products

• [Journal Article] Localized pulmonary crystal-storing histiocytosis complicating pulmonary mucaosa-associated lymphoid tissue lymphoma presenting with multiple mass lesion. (2017)
  • Author(s): Kokuho N, Terasaki Y, Kunugi S, Onda N, Urushiyama H, Terasaki M, Hino M, Gemma A, Hatori T, Shimizu A.
  • Journal Title: Human Pathology Volume: 印刷中 Pages: 180-186
  • DOI: 10.1016/j.humpath.2016.10.028
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Pulmonary mucosa-associated lymphoid tissue lymphoma associated with pulmonary sarcoidosis: a case report and literature review. (2016)
  • Author(s): Kokuho N, Terasaki Y, Urushiyama H, Terasaki M, Kunugi S, Morimoto T, Azuma A, Usuda J, Gemma A, Eishi Y, Shimizu A.
  • Journal Title: Hum Pathol. Volume: 51 Pages: 57-63
  • DOI: 10.1016/j.humpath.2015.12.019
  • Peer Reviewed / Open Access
• [Journal Article] Interstitial lung disease associated with amrubicin chemotherapy in patients with lung cancer: a single institutional study. (2016)
  • Author(s): Miura Y1, Saito Y2, Atsumi K1, Takeuchi S1, Miyanaga A1, Mizutani H, Minegishi Y, Noro R, Seike M, Kunugi S, Kubota K, Gemma A.
  • Journal Title: Jpn J Clin Oncol. 2016 Jul;46(7):674-80. Volume: 46(7) Issue: 7 Pages: 674-680
  • DOI: 10.1093/jjco/hyw043
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of α1 and α2 chains of type IV collagen in early fibrotic lesions of idiopathic interstitial pneumonias and migration of lung fibroblasts. (2015)
  • Author(s): Urushiyama H, Terasaki Y, Nagasaka S, Terasaki M, Kunugi S, Nagase T, Fukuda Y, Shimizu A.
  • Journal Title: Lab Invest Volume: 95 Issue: 8 Pages: 872-885
  • DOI: 10.1038/labinvest.2015.66
  • Peer Reviewed / Open Access
• [Journal Article] Effective Crizotinib schedule for an elderly patient with ALK rearranged non-small-cell lung cancer: a case report. (2015)
  • Author(s): Fukuizumi A, Miyanaga A, Seike M, Kato Y, Nakamichi S, Chubachi K, Matsumoto M, Noro R, Minegishi Y, Kunugi S, Kubota K, Gemma A
  • Journal Title: BMC Res Notes. Volume: 8 Issue: 1 Pages: 165-165
  • DOI: 10.1186/s13104-015-1126-8
  • Peer Reviewed / Open Access
• [Journal Article] Epicardial Catheter Ablation of Ventricular Tachycardia in No Entry Left Ventricle: Mechanical Aortic and Mitral Valves (2015)
  • Author(s): Soejima K, Nogami A, Sekiguchi Y, Harada T, Satomi K, Hirose T, Ueda A, Miwa Y, Sato T, Nishio S, Shirai Y1, Kowase S, Murakoshi N, Kunugi S, Murata H, Nitta T, Aonuma K, Yoshino H
  • Journal Title: Circulation: Arrhythmia and Electrophysiology Volume: 8 Issue: 2 Pages: 381-389
  • DOI: 10.1161/circep.114.002517
  • Peer Reviewed
• [Presentation] 血液疾患に２次性肺胞蛋白症を呈した剖検肺２症例の検討 (2016)
  • Author(s): 功刀 しのぶ, 寺﨑 泰弘, 呉 壮香, 許田 典男, 國保 成暁, 寺崎 美佳, 清水 章
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター 展示棟
• [Book] 集中講義 病理学 (2016)
  • Author(s): 功刀 しのぶ
  • Total Pages: 283
  • Publisher: MEDICAL VIEW