Mesenchymal stem cells cultured in serum-free medium ameliorate experimental renal fibrosis.
| Project/Area Number |
26461229
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Hiroshima University |
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Principal Investigator |
MASAKI TAKAO 広島大学, 病院(医), 教授 (30397913)
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| Co-Investigator(Kenkyū-buntansha) |
中島 歩 広島大学, 原爆放射線医科学研究所, 特任助教 (40448262)
土井 盛博 広島大学, 病院(医), 病院助教 (80626127)
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| Research Collaborator |
KATO YUKIO 広島大学, 名誉教授 (10112062)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 再生医療 / 間葉系幹細胞 / 線維化 / 抗炎症作用 / 腎線維化 / マクロファージ / 無血清培地 |
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| Outline of Final Research Achievements |
The Mechanism underlying the anti-inflammatory effect of mesenchymal stem cells (MSCs) has been gradually elucidated. However, the effect of MSCs cultured in serum-free medium has not been clarified. We investigated the difference between MSCs cultured under 10 % fetal bovine serum (FBS) containing medium and serum-free medium of the therapeutic effects in a rat model of renal fibrosis. After 4 days of unilateral ureteral obstruction (UUO) operation, we injected rat MSCs cultured with 10% FBS containing DMEM (10%MSCs) or serum-free medium (SF-MSCs), or PBS only (PBS) through the rat tail vein. As a results, transplantation of SF-MSCs potently inhibited the infiltration of inflammatory cells and the progression of fibrosis compared with 10%MSCs.
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Report
(4 results)
Research Products
(1 results)
