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Mesenchymal stem cells cultured in serum-free medium ameliorate experimental renal fibrosis.

Research Project

Project/Area Number 26461229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionHiroshima University

Principal Investigator

MASAKI TAKAO  広島大学, 病院(医), 教授 (30397913)

Co-Investigator(Kenkyū-buntansha) 中島 歩  広島大学, 原爆放射線医科学研究所, 特任助教 (40448262)
土井 盛博  広島大学, 病院(医), 病院助教 (80626127)
Research Collaborator KATO YUKIO  広島大学, 名誉教授 (10112062)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords再生医療 / 間葉系幹細胞 / 線維化 / 抗炎症作用 / 腎線維化 / マクロファージ / 無血清培地
Outline of Final Research Achievements

The Mechanism underlying the anti-inflammatory effect of mesenchymal stem cells (MSCs) has been gradually elucidated. However, the effect of MSCs cultured in serum-free medium has not been clarified. We investigated the difference between MSCs cultured under 10 % fetal bovine serum (FBS) containing medium and serum-free medium of the therapeutic effects in a rat model of renal fibrosis. After 4 days of unilateral ureteral obstruction (UUO) operation, we injected rat MSCs cultured with 10% FBS containing DMEM (10%MSCs) or serum-free medium (SF-MSCs), or PBS only (PBS) through the rat tail vein. As a results, transplantation of SF-MSCs potently inhibited the infiltration of inflammatory cells and the progression of fibrosis compared with 10%MSCs.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2016

All Patent(Industrial Property Rights) (1 results)

  • [Patent(Industrial Property Rights)] 生体組織損傷の修復剤および当該修復剤の製造方法2016

    • Inventor(s)
      中島歩、正木崇生、土井盛博、吉田健、加藤幸夫、辻紘一郎
    • Industrial Property Rights Holder
      中島歩、正木崇生、土井盛博、吉田健、加藤幸夫、辻紘一郎
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-256779
    • Filing Date
      2016-12-28
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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