Development of next generation kidney disease biomarker and therapy using microRNA in exosome

• Project/Area Number: 26461231
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Kochi University
• Principal Investigator: Horino Taro 高知大学, 教育研究部医療学系臨床医学部門, 講師 (90448382)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: microRNA / エクソソーム / ループス腎炎 / IgA腎症 / バイオマーカー / バイオマーカ

Outline of Final Research Achievements

Biomarker candidate miRNA was identified from kidney tissue of lupus nephritis model and patients with lupus nephritis or IgA nephropathy.
We clarified that miR-26a is involved in podocyte disorder via expression of Podocin and Synaptopodin with respect to miR-26a, and urinary miR-26a excretion becomes significantly high in lupus nephritis and is useful as a diagnostic biomarker.

Research Products

• [Journal Article] MicroRNAs associated with the development of kidney diseases in humans and animals (2018)
  • Author(s): Ichii O, Horino T.
  • Journal Title: Journal of Toxicologic Pathology Volume: 31 Issue: 1 Pages: 23-34
  • DOI: 10.1293/tox.2017-0051
  • NAID: 130006318235
  • ISSN: 0914-9198, 1881-915X
  • Peer Reviewed / Open Access
• [Journal Article] Urinary exosome-derived microRNAs reflecting the changes of renal function and histopathology in dogs. (2017)
  • Author(s): Ichii O, Ohta H, Horino T, Nakamura T, Hosotani M, Mizoguchi T, Morishita K, Nakamura K, Hoshino Y, Takagi S, Sasaki N, Takiguchi M, Sato R, Oyamada K, Kon Y.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 40340-40340
  • DOI: 10.1038/srep40340
  • NAID: 120005972940
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Serum uric acid level as a risk factor for acute kidney injury in hospitalized patients: a retrospective database analysis using the integrated medical information system at Kochi Medical School hospital. (2016)
  • Author(s): Otomo K, Horino T, Miki T, Kataoka H, Hatakeyama Y, Matsumoto T, Hamada-Ode K, Shimamura Y, Ogata K, Inoue K, Taniguchi Y, Terada Y, Okuhara Y.
  • Journal Title: Clin Exp Nephrol Volume: 20 Issue: 2 Pages: 235-43
  • DOI: 10.1007/s10157-015-1156-5
  • Peer Reviewed
• [Journal Article] Decreased miR-26a expression correlates with the progression of podocyte injury in autoimmune glomerulonephritis. (2014)
  • Author(s): Ichii O, Otsuka-Kanazawa S, Horino T, Kimura J, Nakamura T, Matsumoto M, Toi M, Kon Y
  • Journal Title: PLoS One Volume: 9 Issue: 10 Pages: e110383-e110383
  • DOI: 10.1371/journal.pone.0110383
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Incidence of acute kidney injury among patients with chronic kidney disease: a single-center retrospective database analysis (2015)
  • Author(s): Taro Horino, Yutaka Hatakeyama, Hiromi Kataoka, Tatsuki Matsumoto, Kazu Ode, Yoshiko Shimamura, Koji Ogata, Kosuke Inoue, Yoshinori Taniguchi, Yoshio Terada, Yoshiyasu Okuhara
  • Organizer: Kidney Week, American Society of Nephrology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] MicroRNA-34a Is Up-Regulated in Acute Kidney Injury and by Aging and Regulates PNUTS (PPP1R10) Expression and Apoptosis of Renal Tubular Cells In Vitro and In Vivo (2014)
  • Author(s): Kazu Hamada, Tatsuki Matsumoto, Yoshiko Shimamura, Koji Ogata, Kosuke Inoue, Yoshinori Taniguchi, Taro Horino, Yoshio Terada.
  • Organizer: ASN Kidney Week 2014
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2014-11-11 – 2014-11-16
• [Presentation] Toll-Like Receptor 8 Contributes to Podocyte Injury in Murine Autoimmune Glomerulonephritis (2014)
  • Author(s): Junpei Kimura, Osamu Ichii, Teppei Nakamura, Taro Horino, Saori Otsuka, Yasuhiro Kon.
  • Organizer: ASN Kidney Week 2014
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2014-11-11 – 2014-11-16