a trial for generation of parietal podocytes

• Project/Area Number: 26461241
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Miyazaki Yoichi 東京慈恵会医科大学, 医学部, 教授 (60266690)
• Co-Investigator(Renkei-kenkyūsha): Matsusaka Taiji 東海大学, 医学部・総合医学研究所, 教授 (50317749)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: BMP / 糸球体上皮細胞 / ボーマン嚢上皮細胞 / 糸球体尿細管連関 / podocyte / ＢＭＰ

Outline of Final Research Achievements

After the parietal epithelial cell-specific expression vector (pPEC-rtTA) was constructed and injected into mouse fertilized eggs, we obtained eight founder mice carrying pPEC-rtTA. However, we could not establish activator mouse lines from these mice. Therefore, we examined whether the parietal podocytes were formed in the adult Podocine-rtTA/TetO-Noggin administered Dox for a long time. The results indicated that parietal podocytes were not observed with respect to morphology and expression of several podocyte-markers. In contrast, proximal tubular damages and interstitial edema were intensely seen in the mice.
Hence, in contrast to the prenatal period, inhibition of BMP signal in glomeruli in adulthood cannot induce parietal podocytes.

Research Products

• [Journal Article] Unilateral ureteral obstruction attenuates intrarenal angiotensin Ⅱgeneration induced by podocyte injury. (2015)
  • Author(s): Okabe M,Miyazaki Y,Niimura F,Pastan I,Nishiyama A,Yokoo T,Ichikawa I,Matsusaka T.
  • Journal Title: Am J Physiol Renal Physiol Volume: 308・8 Issue: 8 Pages: F932-F937
  • DOI: 10.1152/ajprenal.00444.2014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Keap1 inhibition attenuates glomerulosclerosis. (2014)
  • Author(s): Miyazaki Y, Shimizu A, Pastan I, Taguchi K, Naganuma E, Suzuki T, Hosoya T, Yokoo T, Saito A, Miyata T, Yamamoto M, Matsusaka T.
  • Journal Title: Nephrol Dial Transplant Volume: 29 Issue: 4 Pages: 783-791
  • DOI: 10.1093/ndt/gfu002
  • Peer Reviewed / Open Access