Clinical usefulness of anti-M-type phospholipase-A-receptor antibodies in patients with membranous nephropathy and the comparison of three quantification methods
| Project/Area Number |
26461242
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 内科 / 免疫学 / 腎臓学 / 膜性腎症 |
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| Outline of Final Research Achievements |
We aimed to establish methods to measure anti-M-type phospholipase A2 receptor (PLA2R) antibodies, specific markers of primary membranous nephropathy (MN), and assess their clinical usefulness. We developed a western blot assay and a cell-based enzyme-linked immunosorbent assay (ELISA). Anti-PLA2R antibodies were detected in 52% of the patients with biopsy-proven primary MN using the western blot. The levels of proteinuria correlated with the anti-PLA2R antibody titers measured by the three methods. Anti-PLA2R antibodies were significantly associated with physicians’ decisions on immunosuppressive treatment. The titers of anti-PLA2R antibodies declined significantly following treatment. These results suggest the usefulness of anti-PLA2R antibody as a diagnostic, prognostic, and surrogate biomarker in primary MN. The three methods proved to be reliable, but their performances differ.
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Report
(5 results)
Research Products
(2 results)
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[Presentation] Development of Cell-Based Enzyme-Linked ImmunoSorbent Assay for the Quantification of Anti-M-type phospholipase-A2 receptor antibodies and Validation of its Clinical Usefulness in Patients with Membranous Nephropathy2014
Author(s)
Yasuhiro Katsumata, Yuko Okamoto, Takahito Moriyama, Manabu Kawamoto, Hirotaka Kaneko, Yasushi Kawaguchi, Takahisa Gono, Masanori Hanaoka, Tomoaki Higuchi, Hidenaga Kawasumi, Keiko Uchida, Kosaku Nitta, Hisashi Yamanaka
Organizer
2014 ACR/ARHP Annual Meeting
Place of Presentation
ボストン（米国）
Year and Date
2014-11-18
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