Elucidation the mechanism of progression on peritoneal sclerosis in peritoneal dialysis
| Project/Area Number |
26461252
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Okayama University |
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Principal Investigator |
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| Research Collaborator |
TSUJI KENZI
TORII AKIKO
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 腹膜中皮細胞 / 腹膜透析 / 細胞療法 / 腹膜再生 |
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| Outline of Final Research Achievements |
In Vitro study, peritoneal mesothelial cells were performed the morphological and qualitative evaluation. The cells used were peritoneal mesothelial cells, with cobblestone morphologic cells (Epi cells) and fibroblast-like peritoneal mesothelial cells (Fib cells). From the result, it is suggested that Fib cells play an important role in peritoneal sclerosis although Epi cells change poorly not only in acidic stimulation but also in sugar stimulation.
In vivo study, Epi cell supernatant significantly improved peritoneal damage and peritoneal adhesion compared to the peritoneal sclerosis positive model, however in Fib cell supernatants, peritoneal deterioration were significantly observed. From these results, it is suggested that secreted factor from peritoneal mesothelial cells is also important. We will also conduct exosome analysis in secreted factors to aim for elucidation of its mechanism.
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Report
(5 results)
Research Products
(15 results)
