Evaluation of 3-factor model (3F) of renal urate transport that may avoid less side-effect of druf-induced hyperuricemia.
| Project/Area Number |
26461258
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Chiba University (2015-2016)
Dokkyo Medical University (2014) |
|---|
Principal Investigator |
Anzai Naohiko 千葉大学, 大学院医学研究院, 教授 (70276054)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
JUTABHA Promsuk 獨協医科大学, 医学部, 助教 (90541748)
大内 基司 獨協医科大学, 医学部, 准教授 (20409155)
大谷 直由 獨協医科大学, 医学部, 助教 (60711819)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SATO Motoyasu 獨協医科大学, 医学部, 助教 (20418891)
OHBAYASHI Tetsuya 鳥取大学, 生命機能研究支援センター, 准教授 (80348804)
|
|---|
| Research Collaborator |
HAYASHI Keitaro
MORITA Asuka
NOHARA Masakatsu
WAKASHIN Hidefumi
TSUCHIYA Go
HORI Takayuki
KANO Yuji
ONIZAWA Nobuyuki
OTSUKA Yusuke
OHNO Yuta
Wempe Michael F.
ENDOU Hitoshi
KIMURA Toru
TAMAI Ikumi
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 尿酸 / 高尿酸血症 / 膜輸送 / トランスポーター / 創薬 |
|---|
| Outline of Final Research Achievements |
This study was aimed to investigate the effectiveness of a novel concept "3-factor model (3F)" and to make an alternative possibility for the development of new drugs that have less side effects regarding drug-induced hyperuricemia. For developing appropriate materials for evaluating this concept that may express triple urate transporters such as URAT1, URATv1, and NPT4, we performed a collaborative study with Dr. Tetsuya Ohbayashi who is a specialist of a mammalian artificial chromosome vector that can be used for introducing multiple genes and succeded to express several kidney-derived genes using his system onto our mouse-derived renal tubular cell named S2. We will use this protocol for further analysis. In addition, we could raise a new animal model that can be used for analyzing the concept of 3F by using pyrazinamide-loading method for creating renal-underexcretion hyperuricemia model.
|
Report
(4 results)
Research Products
(23 results)
-
-
[Journal Article] 4.Kokura K, Kuromi Y, Endo T, Anzai N, Kazuki Y, Oshimura M, Ohbayashi T.2016
Author(s)
Kokura K, Kuromi Y, Endo T, Anzai N, Kazuki Y, Oshimura M, Ohbayashi T.
-
Journal Title
J Gene Med.
Volume: 18
Issue: 10
Pages: 273-281
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Distribution of organic anion transporters NaDC3 and OAT1-3 along the human nephron.2016
Author(s)
Breljak D, Ljubojevic M, Hagos Y, Micek V, Balen Eror D, Vrhovac Madunic I, Brzica H, Karaica D, Radovic N, Kraus O, Anzai N, Koepsell H, Burckhardt G, Burckhardt BC, Sabolic I.
-
Journal Title
Am J Physiol Renal Physiol
Volume: 311
Issue: 1
Pages: F227-F238
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
[Journal Article] Molecular mechanism of the irate-lowering effects of calcium channel blockers.2016
Author(s)
Tsuchiya G, Hori T, Onizawa N, Omani N, Tanaka-Nakadate S, Iseki T, Ouchi M, Hayashi K, Jutabha P, Oba T, Fukuda H, Anzai N.
-
Journal Title
Dokkyo J Med Sci.
Volume: 43
Pages: 23-29
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Ca拮抗薬による尿酸降下作用の解析2014
Author(s)
土屋 豪, 鬼澤 信之, 堀 貴行, Promsuk Jutabha, 大谷 直由, 長谷川 元, 福田 宏嗣, 安西 尚彦
Organizer
第37回日本高血圧学会総会
Place of Presentation
横浜
Year and Date
2014-10-17 – 2014-10-19
Related Report
