• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of neuronal death control target 14-3-3 protein / HMGB1 / Beclin1

Research Project

Project/Area Number 26461282
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurology
Research InstitutionTokyo Medical University

Principal Investigator

Umahara Takahiko  東京医科大学, 医学部, 准教授 (70266477)

Co-Investigator(Kenkyū-buntansha) 秋元 治朗  東京医科大学, 医学部, 教授 (10212440)
羽生 春夫  東京医科大学, 医学部, 主任教授 (10228520)
清水 聰一郎  東京医科大学, 医学部, 講師 (10385031)
宮澤 啓介  東京医科大学, 医学部, 主任教授 (50209897)
橋本 孝朗  東京医科大学, 医学部, 講師 (60266517)
赫 寛雄  東京医科大学, 医学部, 准教授 (70307338)
織田 順  東京医科大学, 医学部, 主任教授 (60459500)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords14-3-3蛋白 / HMGB1 / Beclin1 / 神経細胞死 / 14-3-3蛋白 / HMGB1 / Beclin1 / 脳梗塞 / レビ-小体型認知症 / 血栓 / 頸動脈 / リン酸化TDP43 / オートファジ- / 筋萎縮性側索硬化症 / Beclin 1 / アルツハイマ-病 / TDP-43 / オ-トファジ- / Beclin-1 / 頸動脈プラ-ク
Outline of Final Research Achievements

14-3-3 eta isoform was located in the anterior horn cells of spinal cord of amyotrophic lateral sclerosis. Co-localization with phosphorylated TDP43 was also confirmed. We pointed out the involvement of 14-3-3 protein eta isoform in the process involving phosphorylated TDP - 43 from the nucleus to the cytoplasm and involved in anterior horn cell death. We confirmed the cytoplasmic localization of HMGB1 in neurons around the ischemic core in the acute phase of cerebral infarction. It can contribute as a translational Research of a novel cerebral infarction therapy. Blood HMGB1 concentrations in blood of 29 control subjects, 84 cases of Alzheimer's disease, 8 cases of Parkinson's disease and 25 cases of dementia of Lewy bodies were measured by ELISA method( 5.4, 6.6, 10.7, 8.1 ng / ml). We confirmed localization of autophagy - related protein Beclin 1 in carotid arteriosclerotic lesions and pointed out the involvement of autophagy in arteriosclerosis.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(16 results)

All 2018 2017 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] 14-3-3 protein sigma isoform co-localizes with phosphorylated α-synuclein in Lewy bodies and Lewy neurites in patients with Lewy body disease2018

    • Author(s)
      Wakabayashi Kaori、Umahara Takahiko、Hirokawa Katsuiku、Hanyu Haruo、Uchihara Toshiki
    • Journal Title

      Neuroscience Letters

      Volume: 674 Pages: 171-175

    • DOI

      10.1016/j.neulet.2018.03.013

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 14-3-3 eta isoform colocalizes TDP-43 on the coarse granules in the anterior horn cells of patients with sporadic amyotrophic lateral sclerosis,2016

    • Author(s)
      Umahara T, Uchihara T, Shibata N, Nakamura A, Hanyu H
    • Journal Title

      Brain Reserch

      Volume: 1646 Pages: 132-132

    • DOI

      10.1016/j.brainres.2016.05.051

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 脳梗塞剖検脳における急性期および慢性期でのHMGB1局在の差異2017

    • Author(s)
      馬原孝彦、山川仁子、櫻井博文、羽生春夫
    • Organizer
      第51回日本成人病(生活習慣病)学会学術集会
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2017-01-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 免疫組織化学から認知症治療開発へ向けての情報提示2017

    • Author(s)
      馬原孝彦
    • Organizer
      日本老年医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 14-3-3 sigma isoform co-localizes with phosphorylated α-synuclein in Lewy bodies and Lewy neurites of the patients with Lewy body disease2017

    • Author(s)
      Umahara T, Wakabayashi K Hirokawa K, Hanyu H , Uchihara T
    • Organizer
      XXIII World Congress of Neurology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Immunohistochemical Examination of Human Mural Thrombus Associated with Carotid Plaques2016

    • Author(s)
      Takahiko Umahara, Haruo Hanyu, Toshiki Uchihara
    • Organizer
      the 9th Congress of the Asian-Pacific Society on Thrombosis and Hemostasis
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 高齢者脳動脈および脳実質病変でのHMGB1の発現様式2016

    • Author(s)
      馬原孝彦 廣瀬大輔 深沢雷太 清水聰一郎 山川仁子 平尾健太郎 金高秀和 櫻井博文 羽生春夫 内原俊記
    • Organizer
      第58回日本老年医学会学会学術集会
    • Place of Presentation
      石川県金沢市
    • Year and Date
      2016-06-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Localizaton of Beclin-1 in human main cerebral and caroed arteries2016

    • Author(s)
      Takahiko Umahara, Shoichiro Shimizu, Kentaro Hirao, Haruo Hanyu, Toshiki Uchihara
    • Organizer
      第57回日本神経学会学術大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report
  • [Presentation] 急性期脳梗塞剖検脳でのHMGB 1の発現様式-抗体治療の基礎的研究として-2016

    • Author(s)
      馬原 孝彦
    • Organizer
      第41回日本脳卒中学会総会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-04-14
    • Related Report
      2015 Research-status Report
  • [Presentation] 脳細動脈におけるマクロファ-ジとHMGB 1蛋白の局在2016

    • Author(s)
      馬原 孝彦
    • Organizer
      第50回日本成人病(生活習慣病)学会学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2016-01-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Immunolocalization of 14-3-3 proteins in atheroslerotic lesions of human carotid and main cerebral arteries2015

    • Author(s)
      馬原 孝彦
    • Organizer
      International Association of Gerontology and Geriatrics Asia/Oceania 2015 Congress
    • Place of Presentation
      チェンマイ タイ
    • Year and Date
      2015-10-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] オ-トファジ-関連物質Beclin 1の頸動脈硬化病変での発現-第二報-2015

    • Author(s)
      馬原 孝彦
    • Organizer
      第57回日本老年医学会学会学術集会
    • Place of Presentation
      横浜市
    • Year and Date
      2015-06-14
    • Related Report
      2015 Research-status Report
  • [Presentation] 主幹脳動脈および頸動脈病変でのHMGB1蛋白の局在意義-続報-2015

    • Author(s)
      馬原 孝彦
    • Organizer
      第56回日本神経学会学術大会
    • Place of Presentation
      新潟市
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report
  • [Presentation] ダメ-ジ関連分子パタ-ンHMGB 1蛋白の局在様式-脳卒中治療応用への基礎的検討-2015

    • Author(s)
      馬原 孝彦
    • Organizer
      日本脳卒中学会総会
    • Place of Presentation
      広島県広島市 リーガロイヤルホテル広島
    • Year and Date
      2015-03-26 – 2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 頸動脈超音波検査法は頸動脈プラ-クの免役組織化学所見を反映できるか?―3D Tomogfaphic Ultrasound Imaging(多断面表示)での試みー2015

    • Author(s)
      山川 仁子
    • Organizer
      日本成人病(生活習慣病9学会学術集会
    • Place of Presentation
      東京都千代田区 都市センタ-ホテル
    • Year and Date
      2015-01-10 – 2015-01-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 障害関連分子HMGB1の分布-「炎症と認知機能障害」の基礎的検討として-2014

    • Author(s)
      馬原 孝彦
    • Organizer
      日本認知症学会総会学術集会
    • Place of Presentation
      神奈川県横浜市 パシフィコ横浜
    • Year and Date
      2014-11-29 – 2014-12-01
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi