| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
Poly (ADP Ribose) Polymerase-1 (PARP1) binds to the NACP-Rep1 polymorphic site upstream of α-Synuclein (αSyn) gene and negatively regulates αSyn expression. When the construction of the plasmid carrying the PARP1 and miRNA-124 (miR-124) were cotransfected into cultured cells, miR-124 significantly reduced PARP1 expression. In order to specify the inhibitory mechanism, we mutated the predicted target sequence of miR-124 on the PARP1 mRNA 3’ untranslated region in the plasmid by substitution or deletion of the nucleotides. The mutation partly eliminated the inhibition of PARP1 expression by miR-124. These results suggest that miR-124 inhibits PARP1 expression by binding not only directly to PARP1 mRNA 3’untranslated region, but also to another target mRNA.
|
