| Project/Area Number |
26461290
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Gifu University |
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Principal Investigator |
Kimura Akio 岐阜大学, 大学院医学系研究科, 准教授 (00362161)
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| Co-Investigator(Kenkyū-buntansha) |
矢野 竹男 三重大学, 地域イノベーション学研究科, 教授 (00569197)
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| Research Collaborator |
YOSHIKURA Nobuaki 岐阜大学, 医学部附属病院, 助教
KOUMURA Akihiro 岐阜市民病院, 神経内科, 部長
TAKEMURA Masao 岐阜大学, 医学部附属病院, 検査技師
INUZUKA Takashi 岐阜大学, 大学院医学系研究科, 教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 抗血管内皮抗体 / 脳小血管病変 / Annexin A2 / Amyloid β / Hexokinase 1 / 大脳微小血管 / 大脳白質病変 / アネキシンA2 / 脳小血管病 / バイオーカー / 神経病態免疫学 / 自己抗体 |
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| Outline of Final Research Achievements |
We detected the anti-endothelial cell autoantibodies in sera and cerebrospinal fluid (CSF) from patients with neurological diseases by 2-dimensional immunoblotting or immunoprecipitation using primary cultured human brain microvascular endothelial cells as the antigen source. Subsequently, we analyzed the target antigens of these autoantibodies by liquid chromatography-tandem mass spectrometry. We also analyzed the association between the titer of identified anti-endothelial cell autoantibodies and the severity of cerebral small vessel disease. We detected the autoantibody against hexokinase 1, which is a mitochondrial outer membrane protein and play a role for regulation of energy production and apoptosis, in sera from the patient with severe small vessel disease. We also found that the serum anti-Annexin A2 autoantibodies and CSF anti-amyloid β autoantibodies are associated with the severity of cerebral small vessel disease in patients with Alzheimer's disease.
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