• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of non-canonical T1R3 homomeric sweet receptor in adipogenesis and glucose transport

Research Project

Project/Area Number 26461325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionGunma University

Principal Investigator

Shibata Hiroshi  群馬大学, 生体調節研究所, 准教授 (20235584)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords甘味受容体 / 脂肪細胞 / 微小管 / Rho / サイトカイン / マクロファージ / 炎症性サイトカイン / TNFα / T1R3 / グルコース輸送 / MCP-1 / 脂肪分化 / Gタンパク / FoxO1
Outline of Final Research Achievements

3T3-L1 cells express a functional sweet taste receptor as a T1R3 homomer that negatively regulates adipogenesis by a Gαs-mediated but cAMP-independent mechanism. In this study, we show that stimulation of this receptor induced microtubules disassembly in 3T3-L1 preadipocytes, which was attenuated by overexpression of the dominant-negative mutant of Gαs (Gαs-G226A) and mimicked by overexpression of the constitutively active mutant of Gαs (Gαs-Q227L). Sweetener also activated RhoA and Rho-associated kinase (ROCK), which was attenuated with by knockdown of GEF-H1, a microtubule-localized RhoGEF. Overexpression of the dominant-negative mutant of RhoA (RhoA-T19N) blocked sweetener-induced dephosphorylation of Akt and repression of PPARγ and C/EBPα in the early phase of adipogenic differentiation. Thus, the T1R3 homomeric sweet taste receptor negatively regulates adipogenesis through Gαs-mediated microtubule disassembly and consequent activation of the Rho/ROCK pathway.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2017 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Int'l Joint Research] University of Illinois, Chicago(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Illinois at Chicago(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] T1R3 homomeric sweet taste receptor regulates adipogenesis through Gas-mediated microtubules disassembly and Rho activation in 3T3-L1 cells2017

    • Author(s)
      Y. Masubuchi, Y. Nakagawa, J. Medina, M. Nagasawa, I. Kojima, M. M. Rasenick, T. Inagaki, H. Shibata
    • Journal Title

      PLOS ONE

      Volume: - Issue: 5 Pages: e0176841-e0176841

    • DOI

      10.1371/journal.pone.0176841

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Prolonged insulin stimulation down-regulates GLUT4 through oxidative stress-mediated retromer inhibition by a protein kinase CK2-dependent mechanism in 3T3-L1 adipocytes2014

    • Author(s)
      Ma J, Nakagawa Y, Kojima I, Shibata H
    • Journal Title

      J Biol Chem

      Volume: 289 Pages: 133-142

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Glucose promotes its own metabolism by acting on the cell-surface glucose-sensing receptor T1R32014

    • Author(s)
      Nakagawa Y, Ohtsu Y, Nagasawa M, Shibata H, Kojima I
    • Journal Title

      Endocr J

      Volume: 61 Pages: 119-131

    • NAID

      130004718269

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] マクロファージに発現するT1R3ホモマー甘味受容体の機能2017

    • Author(s)
      李龍飛,ヨハン・メディナ,長澤雅裕,稲垣毅,柴田宏
    • Organizer
      第60回日本糖尿病学会年次学術集会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2017-05-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Two Divergent Signals from the Gs-Coupled T1R3 Homomeric Sweet Taste Receptor Converge at FoxO1 to Inhibit Adipogenesis in 3T3-L1 Cells2015

    • Author(s)
      Yosuke Masubuchi, Itaru Kojima, Hiroshi Shibata
    • Organizer
      American Diabetes Association 75th Scientific Sessions
    • Place of Presentation
      ボストンコンベンション・エグジビションセンター(アメリカ合衆国・マサチューセッツ州・ボストン市)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Gs共役T1R3ホモマー甘味受容体による脱アセチル化酵素SIRT2活性化と脂肪分化抑制2015

    • Author(s)
      増渕洋祐,小島至,柴田宏
    • Organizer
      第58回日本糖尿病学会年次学術集会
    • Place of Presentation
      海峡メッセ(山口県・下関市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Gs共役型T1R3ホモマー甘味受容体による微小管脱重合とRho活性化を介したインスリン依存性グルコース輸送の抑制2014

    • Author(s)
      増渕洋祐,中川祐子,馬金輝,長澤雅裕,小島至,柴田宏
    • Organizer
      第57回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-22 – 2014-05-24
    • Related Report
      2014 Research-status Report
  • [Remarks] 群馬大学生体調節研究所 細胞調節分野

    • URL

      http://www.imcr.gunma-u.ac.jp/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-02-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi