| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We determined the comprehensive mRNA expression profiles in the ghrelin-producing cell line, MGN3-1 cells. By examining the ligands for the highly expressed GPCR, we found that prostaglandin E2 and tryptophan stimulated ghrelin secretion via EP4 receptor and GPR142, respectively. Except for GPCR, we found that IL-1b significantly suppressed ghrelin mRNA expression in MGN3-1 cells, at least partly through NF-kB pathway. We found that ghrelin-producing cells highly incorporated long-chain fatty acids. The high incorporation of long-chain fatty acids to the cells, which was provided by high levels of Acsl1, contributed to the efficient acylation of ghrelin.
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