| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, I start to develop the imaging method for measuring a pancreatic beta cell mass by using fluorescent probe. A fluorescent probe with exendin frame was prepared. The probe targeted a glucagon like-peptide 1 receptor (GLP-1R) which highly expressed in pancreatic beta cells. To evaluate the probe properties, binding affinity study and binding study for cells, and immunostaining study of pancreatic slices were performed. The specific binding for GLP-1R was shown by binding affinity study. And the binding for cell with GLP-1R was shown by fluorescent image analysis and flow cytometry analysis.
These studies showed that the fluorescent probe with exendin has a possibility for imaging the pancreatic beta cell and analyzing the mass.
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