Development for pancreatic beta cell imaging method by using a fluorescent probe
Project/Area Number |
26461334
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Metabolomics
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 膵島イメージング / 蛍光イメージング / イメージング / 蛍光 / 糖尿病 / 蛍光プローブ / 膵β細胞 |
Outline of Final Research Achievements |
In this study, I start to develop the imaging method for measuring a pancreatic beta cell mass by using fluorescent probe. A fluorescent probe with exendin frame was prepared. The probe targeted a glucagon like-peptide 1 receptor (GLP-1R) which highly expressed in pancreatic beta cells. To evaluate the probe properties, binding affinity study and binding study for cells, and immunostaining study of pancreatic slices were performed. The specific binding for GLP-1R was shown by binding affinity study. And the binding for cell with GLP-1R was shown by fluorescent image analysis and flow cytometry analysis. These studies showed that the fluorescent probe with exendin has a possibility for imaging the pancreatic beta cell and analyzing the mass.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Development of 111In-labeled exendin(9-39) derivatives for single-photon emission computed tomography imaging of insulinoma.2017
Author(s)
Hiroyuki Kimura, Hirokazu Matsuda, Yu Ogawa, Hiroyuki Fujimoto, Kentaro Toyoda, Naotaka Fujita, Kenji Arimitsu, Keita Hamamatsu, Yusuke Yagi, Masahiro Ono, Nobuya Inagaki, Hideo Saji.
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Journal Title
Bioorganic and Medicinal Chemistry
Volume: 25
Issue: 4
Pages: 1406-1412
DOI
Related Report
Peer Reviewed
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[Journal Article] Synthesis and evaluation of 18F-labeled mitiglinide derivatives as positron emission tomography tracers for β-cell imaging.2014
Author(s)
H Kimura, H Matsuda H Fujimoto, K Arimitsu, K Toyoda, E Mukai, H Nakamura, Y Ogawa, M Takagi, Mo Ono, N Inagaki, H Saji.
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Journal Title
Bioorganic & Medicinal Chemistry
Volume: 22
Issue: 13
Pages: 3270-3278
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Fujita, N., Fujimoto, H., Hamamatsu, K., Kimura, H., Sano, K., Toyoda, K., Saji, H., Inagaki, N2016
Author(s)
Fujita, N., Fujimoto, H., Hamamatsu, K., Kimura, H., Sano, K., Toyoda, K., Saji, H., Inagaki, N
Organizer
American Diabetes Association 76th Scientific sessions
Place of Presentation
New Orleans LA (USA)
Year and Date
2016-06-10
Related Report
Int'l Joint Research
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