Evaluation of the association between intracerebral insulin action and cognitive impairment in animal models of Alzheimer disease

• Project/Area Number: 26461341
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Kumamoto University
• Principal Investigator: Kawashima Junji 熊本大学, 大学院生命科学研究部(医), 助教 (70467984)
• Co-Investigator(Kenkyū-buntansha): 荒木 栄一 熊本大学, 大学院生命科学研究部(医), 教授 (10253733)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 認知症 / インスリン抵抗性 / 認知機能 / アルツハイマー型認知症 / 認知機能障害

Outline of Final Research Achievements

We generated Alzheimer disease model mice with insulin resistance by mating IRS1 knockout (IRS1KO) mice with the Alzheimer disease model mice, APP23 transgenic (APP23Tg) mice or human mutant APP knock-in mice (NL/F KI), respectively.
It was not significant difference in cognitive function determined by Y maze test between APP23Tg(-)-IRS1 wild type (WT) mice, APP23Tg(+)-IRS1WTmice, APP23Tg(-)-IRS1KO mice and APP23Tg(+)-IRS1KO mice.
It was not significant difference in cognitive function determined by Y maze test between APPWT-IRS1WT mice, NL/F homo KI-IRS1WT mice, APPWT-IRS1KO mice and NL/F homo KI-IRS1KO mice.