GDP-bound Rab27a regulates endocytosis by mediating vATPase-dependent regulation of pH
| Project/Area Number |
26461342
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIZAKI Toshimasa 大分大学, 医学部, 教授 (70293876)
TERABAYASHI Takeshi 大分大学, 医学部, 助教 (40452429)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 糖尿病 / インスリン分泌 / Rab27a / エンドサイトーシス / 膵B細胞 / Gタンパク質 / メンブレントラフィック / エキソサイトーシス / インスリン / シグナル伝達 / プロテオーム / タンパク質 / 低分子量Gタンパク質 |
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| Outline of Final Research Achievements |
Impaired insulin secretion in response to glucose plays an important role in the pathogenesis of diabetes mellitus. Although endocytosis of secretory membranes after insulin release is essential for the tight control of insulin secretion, knowledge about this process in pancreatic beta-cells is still limited. Here, I searched for GDP-bound Rab27a interacting proteins and identified proton pumps. I found that GDP-bound Rab27a promotes the formation of the proton pump complex, which is required for its activity. These results suggest that GDP-bound Rab27a regulates endocytosis via acidification in endocytosed vesicles.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] PI3K regulates endocytosis after insulin secretion via signaling crosstalk between Arf6 and Rab27a.2016
Author(s)
Yamaoka M, Ando T, Terabayashi T, Okamoto M, Takei M, Nishioka T, Kaibuchi K, Matsunaga K, Ishizaki R, Izumi T, Niki I, Ishizaki T, Kimura T
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Journal Title
J. Cell Sci.
Volume: 129
Pages: 637-649
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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