| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We recently identified a novel transcriptional cofactor HELZ2 that binds to the nuclear receptor PPARγ and reported that HELZ2-deficient mice were resistant to high fat diet-induced obesity. We also identified THRAP3 and SFPQ as proteins binding to HELZ2 using immunoprecipitation/TOFMS analyses. In the present study, we found that THRAP3 knockdown in 3T3-L1 cells altered alternative splicing (AS) patterns of genes involved in lipid metabolism. Furthermore, knockdown of SFPQ suppressed adipocyte differentiation by altering AS of transcription factors in addition to the expression of transcription factors in the early differentiation. These findings together suggest that HELZ2-binding proteins could play important roles by regulating AS in the differentiation of white adipocytes.
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