| Project/Area Number |
26461367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Saitama Medical University |
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Principal Investigator |
INOUE Ikuo 埼玉医科大学, 医学部, 教授 (60232526)
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| Co-Investigator(Kenkyū-buntansha) |
中野 貴成 埼玉医科大学, 医学部, 講師 (20406474)
竹中 康浩 埼玉医科大学, 医学部, 研究員 (20586789)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | PPARγ1 / PPARγ2 / プロモータ / スプライシングバリアント / 脂肪細胞分化誘導 / ノックアウト(KO)ホモマウス / PPARgamma / ノックアウト(KO)マウス / 選択的スプライシングバリアント / 胎生致死 / ノックアウトマウス / 転写因子 / CRISPR-Cas9システム |
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| Outline of Final Research Achievements |
We discovered a new splicing valiant (SV) transcribed from exon C of PPARγ1, that is previously reported promoter different from exon B of PPARγ1 (Fig.1A)(Takahashi S, Inoue I, et al. J Atheroscler Thromb 17:73-83, 2010). It proved that a new splicing valiant (SV) is involved in induction of adipocyte differentiation as well as PPARγ2(Y Takenaka, I Inoue: PLoS One 8: e 65583, 2013). We established this site specific knockout (KO) homo mice (Fig.1A) and was found to be embryonic lethal (Fig.1C). On the other hand, conventionally known exon A1 specific KO homo mice were growing normally until 18.5th fertilization (Fig.2).
Currently, exon C specific adipocyte-specific conditional gene KO mouse has also been prepared.
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