Plasticity of breast cancer, origin of hormonal therapy resistance and cancer stemness.
| Project/Area Number |
26461373
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ER発現 / ホルモン療法耐性 / シグナル経路 / 癌幹細胞性 / 乳がん / エストロゲン / MCF-7細胞 / T47D細胞 / シグナル伝達 / エピゲノム制御 / 乳癌 / アロマターゼ阻害剤 / GFP / アンドロゲン |
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| Outline of Final Research Achievements |
We have established 8 kinds of hormonal therapy resistant cell lines from ER-positive breast cancer cells which were stably introduced ERE-GFP reporter plasmid. The analysis of these cell lines revealed individual molecular mechanisms of hormonal therapy resistance. Then, we analyzed cancer stemness property of these resistant cell lines (AI-resistant and fulvestrant resistant cell lines) in terms of sphere formation, side population, CD44/CD24 expression etc. The resistant cell lines which have high ER expression showed high cancer stemness property, but cell lines which lost ER expression indicated low cancer stemness marker expression. These findings will provide useful information to develop new therapeutic strategy for metastatic breast cancer.
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Report
(4 results)
Research Products
(62 results)
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[Journal Article] Prognostic significance of immunohistochemical expression of the ubiquitin ligase carboxyl terminus of Hsc70-interacting protein (CHIP) in postmenopausal women with invasive breast cancer2016
Author(s)
Kurozumi S, Yamaguchi Y, Hayashi S, Hiyoshi H, Suda T, Matsumoto H, Takei H, Horiguchi J, Oyama T, Takeyoshi I, Kurosumi M
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Journal Title
Cancer Med
Volume: 5(8)
Issue: 8
Pages: 1873-1882
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Increased androgen receptor activity and cell proliferation in aromatase inhibitor-resistant breast carcinoma2014
Author(s)
Fujii R, Hanamura T, Suzuki T, Gohno T, Shibahara Y, Niwa T, Yamaguchi Y, Ohnuki K, Kakugawa Y, Hirakawa H, Ishida T, Sasano H, Ohuchi N, Hayashi S
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Journal Title
J. Steriod Biochem. Mol. Biol
Volume: 144
Pages: 513-522
DOI
Related Report
Peer Reviewed
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[Presentation] 内分泌療法耐性のメカニズム2014
Author(s)
林慎一
Organizer
第52回日本乳癌治療学会学術集会
Place of Presentation
横浜（パシフィコ横浜）
Year and Date
2014-08-28 – 2014-08-30
Related Report
Invited
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