Involvement of transcription elongation factor in transcriptional regulation by thyroid hormone and its receptor

Research Project

Project/Area Number	26461378
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Endocrinology
Research Institution	Hamamatsu University School of Medicine
Principal Investigator	Matsushita Akio 浜松医科大学, 医学部附属病院, 助教 (50402269)
Co-Investigator(Kenkyū-buntansha)	佐々木茂和浜松医科大学, 医学部附属病院, 講師 (20303547)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	甲状腺ホルモン / 核内受容体 / 転写制御 / 転写伸長反応 / 受容体 / 転写調節 / 転写伸長因子
Outline of Final Research Achievements	Thyroid hormone (T3) binds to the thyroid hormone receptor (TR) in the nucleus and exerts hormonal action by regulating the transcriptional activity of the target gene. From the analysis of factors involved in transcriptional regulation of TR, we found that cyclin dependent kinase 9 (CDK9), which is a component of the positive transcriptional elongation factor (P-TEFb) that promotes mRNA synthesis reaction, binds to TR. By recruiting CDK9, TR promotes the transcriptional elongation reaction and enhances the transcriptional activity of the target gene.