| Project/Area Number |
26461379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Nagoya University |
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Principal Investigator |
Suga Hidetaka 名古屋大学, 医学部附属病院, 助教 (20569818)
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| Co-Investigator(Kenkyū-buntansha) |
長崎 弘 藤田保健衛生大学, 医学部, 教授 (30420384)
大磯 ユタカ 名古屋大学, 医学系研究科, 教授 (40203707)
有馬 寛 名古屋大学, 医学系研究科, 教授 (50422770)
萩原 大輔 名古屋大学, 医学部附属病院, 病院助教 (70710086)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 疾患特異的iPS細胞 / 家族性中枢性尿崩症 / 神経変性疾患 / 疾患モデル / 小胞体ストレス / オートファジー |
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| Outline of Final Research Achievements |
One of the goals for our study is establishing in vitro disease model using disease specific iPS cells. In this study, we chose familial neurohypophysial diabetes insipidus (FNDI) as a degenerative neuronal disease model. We already have made a mouse model for FNDI, an autosomal dominant disease characterized by progressive polyuria, which usually manifests several months or years after birth in human patients. We induced disease specific mouse iPS cell lines from those FNDI model mice, and confirmed the successful induction into hypothalamic vasopressin neurons from disease specific mouse iPS cells after the technical improvement for in vitro differentiation methods. Importantly, those induced vasopressin neurons contained round shaped aggregates in the cytoplasm, which is the same phenomenon in the hypothalamus of FNDI mice. We are planning to use this induced vasopressin neuron as a disease model for further pathologic analyses and drug screening.
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