| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In order to clarify the spatiotemporal-specific expression mechanism of aromatase involved in sexual differentiation of the brain, I performed functional analysis of Deaf1, a transcription factor responsible for the regulation of the aromatase expression in the brain. The diencephalic region was excised from the fetal mouse brain, and a nuclear extract was prepared. To obtain a sample containing protein interacting with Deaf1, immunoprecipitation reaction was carried out using an antibody recognizing endogenous Deaf1 protein. The obtained sample was subjected to SDS-PAGE, and protein band was excised. LC-MS / MS analysis of the sample revealed that Transcriptional Intermediary Factor-1β (TIF-1β) is as one of the molecule interacting with the Deaf1 protein.
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