Analysis of protein regulating brain-specific aromatase gene expression through proteomics
Project/Area Number |
26461389
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Endocrinology
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Research Institution | Fukuoka University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | ステロイドホルモン / 神経細胞 / 性分化 / 転写制御 / 転写調節因子 / アロマターゼ / 中枢神経系 |
Outline of Final Research Achievements |
In order to clarify the spatiotemporal-specific expression mechanism of aromatase involved in sexual differentiation of the brain, I performed functional analysis of Deaf1, a transcription factor responsible for the regulation of the aromatase expression in the brain. The diencephalic region was excised from the fetal mouse brain, and a nuclear extract was prepared. To obtain a sample containing protein interacting with Deaf1, immunoprecipitation reaction was carried out using an antibody recognizing endogenous Deaf1 protein. The obtained sample was subjected to SDS-PAGE, and protein band was excised. LC-MS / MS analysis of the sample revealed that Transcriptional Intermediary Factor-1β (TIF-1β) is as one of the molecule interacting with the Deaf1 protein.
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Report
(5 results)
Research Products
(8 results)
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[Journal Article] Aromatase knockout mice reveal an impact of estrogen on drug-induced alternation of murine electrocardiography parameters2015
Author(s)
Kurokawa, J., Sasano, T., Kodama, M., Li, M., Ebana, Y., Harada, N., Honda, S., Nakaya, H., Furukawa, T
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Journal Title
J. Toxicol. Sci.
Volume: 40
Pages: 339-348
NAID
Related Report
Peer Reviewed
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