Functional analysis of Anamorsin in iron metabolism in vivo
Project/Area Number |
26461401
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Osaka University |
Principal Investigator |
HAMANAKA Yuri 大阪大学, 医学部附属病院, 医員 (50722287)
|
Co-Investigator(Kenkyū-buntansha) |
金倉 譲 大阪大学, 医学系研究科, 教授 (20177489)
柴山 浩彦 大阪大学, 医学系研究科, 講師 (60346202)
織谷 健司 大阪大学, 医学系研究科, 准教授 (70324762)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | アナモルシン / 鉄代謝 / アナモルシン コンディショナルノックアウトマウス / アルブミンCreトランスジェニックマウス / ヘプシジン / コンディショナルノックアウトマウス |
Outline of Final Research Achievements |
Anamorsin (AM) has been reported to be involved in intracellular iron metabolism, however, its involvement in iron metabolism in vivo is still unknown. Because AM-deficient mice are embryonic lethal, it is difficult to analyze the functions of AM in adult mice. To overcome the embryonic lethality of AM-deficient mice, AM conditional knockout mice using Cre-loxP system were generated and crossed with Alb-Cre Tg mice to generate hepatocyte specific AM-deficient mice (Alb-Cre, AM Flox/Flox mice). In the liver cells of the Alb-Cre, AM Flox/Flox mice, enlargement of the nucleus of hepatocytes and nuclear inclusion bodies in some hepatocytes were observed. The accumulation of some substances (not specified yet) in the cytoplasm was also observed, which suggested that AM might have some roles in some substances metabolism. Considering the possibility of being glycogen or iron, we will clarify the substances in the future study.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] The anti-apoptotic gene Anamorsin is essential for both autonomous and extrinsic regulation of murine fetal liver hematopoiesis.2014
Author(s)
Tanimura A, Shibayama H, Hamanaka Y, Fujita N, Ishibashi T, Sudo T, Yokota T, Ezoe S, Tanaka H, Matsumura I, Oritani K, Kanakura Y.
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Journal Title
Exp Hematol.
Volume: 42
Issue: 5
Pages: 410-422
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Anamorsin, a novel caspase-3 substrate in neurodegeneration.2014
Author(s)
Yun N, Lee YM, Kim C, Shibayama H, Tanimura A, Hamanaka Y, Kanakura Y, Park IS, Jo A, Shin JH, Ju C, Kim WK, Oh YJ.
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Journal Title
J Biol Chem
Volume: 289
Issue: 32
Pages: 22183-22195
DOI
Related Report
Peer Reviewed
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[Presentation] ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias.2015
Author(s)
Ishibashi T,Yokota T, Tanaka H, Ichii1 M, Sudo T, Satoh Y, Doi Y, Tanimura A, Hamanaka Y, Ezoe S, Shibayama H, Oritani K, Kanakura Y.
Organizer
The American Society of Hematology 57th Annual Meeting
Place of Presentation
Orange County Convention Center, Orland, FL, USA
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] Endothelial cell-selective adhesion molecule is a novel human hematopoietic stem cell markerassociated with a subset of human leukemias.2015
Author(s)
Ishibashi T,Yokota T, Tanaka H, Ichii1 M, Sudo T, Satoh Y, Doi Y, Tanimura A, Hamanaka Y, Ezoe S, Shibayama H, Oritani K, Kanakura Y.
Organizer
The 44th Annual Scientific Meeting of International Society of Experimental Hematology (ISEH)
Place of Presentation
Kyoto International Conference Center, Kyoto Japan
Year and Date
2015-09-17
Related Report
Int'l Joint Research
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[Presentation] An Anti-Apoptotic Molecule, Anamorsin, Is Upregulated in Anemia and Essential for Imperative Erythropoiesis.2015
Author(s)
Akira Tanimura, Hirohiko Shibayama, Yuri Hamanaka, Natsuko Fujita, Yasuhiro Nagate, Tomohiko Ishibashi, Michiko Ichii, Takafumi Yokota, Sachiko Ezoe, Kenji Oritani, and Yuzuru Kanakura
Organizer
第6回 JSH International Symposium 2015
Place of Presentation
軽井沢プリンスホテル, 長野, 北佐久郡
Year and Date
2015-05-22
Related Report
Int'l Joint Research
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[Presentation] Anamorsin Overexpression Leads to Dysregulation of Lipopolysaccharide-Stimulated B Cell Proliferation through Ras Signaling2014
Author(s)
Yuri Hamanaka, Hirohiko Shibayama, Akira Tanimura, Takafumi Yokota, Sachiko Ezoe, Michiko Ichii, Norimitsu Saito, Keiko Matsui, Takao Sudo, Tomohiko Ishibashi, Yukiko Doi, Yasuhiro Nagate, Masako Takemoto, Kenji Oritani and Yuzuru Kanakura
Organizer
The American Society of Hematology 56th Annual Meeting
Place of Presentation
San Francisco (USA)
Year and Date
2014-12-08
Related Report
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[Presentation] An Anti-Apoptotic Molecule, Anamorsin, Is Upregulated in Anemia and Essential for Imperative Erytropoiesis2014
Author(s)
Akira Tanimura, Hirohiko Shibayama, Hamanaka Yuri, Natsuko Fujita, Nagate Yasuhiro, Tomohiko Ishibashi, Norimitsu Saitoh, Michiko Ichii, Takafumi Yokota, Sachiko Ezoe, Kenji Oritani and Yuzuru Kanakura
Organizer
he American Society of Hematology 56th Annual Meeting
Place of Presentation
San Francisco (USA)
Year and Date
2014-12-08
Related Report
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