| Project/Area Number |
26461403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
Kato Hisashi 大阪大学, 医学系研究科, 助教 (20705214)
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| Co-Investigator(Kenkyū-buntansha) |
柏木 浩和 大阪大学, 医学系研究科, 講師 (10432535)
冨山 佳昭 大阪大学, 医学部附属病院, 准教授 (80252667)
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| Co-Investigator(Renkei-kenkyūsha) |
Tomiyama Yoshiaki 大阪大学, 医学部附属病院・輸血部, 准教授 (80252667)
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| Research Collaborator |
Ichii Michiko 大阪大学, 大学院医学系研究科・血液・腫瘍内科, 寄付講座助教 (30633010)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | インテグリンαIIbβ3 / Inside-Outシグナル / CalDAG-GEFI / 血小板 / インテグリン活性化 / 血栓 / inside-outシグナル |
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| Outline of Final Research Achievements |
Platelet fibrinogen receptor integrin αIIbβ3 is essential for hemostasis and its activation state is tightly regulated by inside-out signaling. So far, the detailed mechanism how inside-out signaling induces activation of αIIbβ3 is still obscure. In this study, we determined the molecules which are important for inside-out signaling in the megakaryocytic cell line CMK and the patient with CalDAG-GEFI deficiency who has been suffering severe bleeding problems. However, platelets with CalDAG-GEFI deficiency showed mildly impaired αIIbβ3 activation. And we found the delayed αIIbβ3 activation in CalDAG-GEFI deficient platelets is related to severe bleeding symptoms. Our results indicate the importance of CalDAG-GEFI and αIIbβ3 activation kinetics for normal hemostasis (Blood 2016).
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