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Functional analysis of the newly identified macrophage differentiation-inducing factor IL-32

Research Project

Project/Area Number 26461407
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hematology
Research InstitutionKumamoto University

Principal Investigator

Suzu Shinya  熊本大学, エイズ学研究センター, 教授 (80363513)

Co-Investigator(Renkei-kenkyūsha) Noyori Osamu  熊本大学, エイズ学研究センター/国際先端医学研究拠点施設, 特定事業研究員 (30737151)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞分化 / マクロファージ / サイトカイン / IL-32 / 分化 / M-CSF
Outline of Final Research Achievements

M-CSF stimulates differentiation/survival of macrophages (MF) and induces anti-inflammatory M2 but not pro-inflammatory M1 MF. Recently, another cytokine IL-32 was reported to promote MF differentiation. Here, we found that M-CSF has additive/inhibitory effects on IL-32 activities. When added to M-CSF-MF, these cytokines supported MF survival, which was enhanced by their combination. However, they had opposed effects on HIV-1 replication; stimulated by M-CSF and inhibited by IL-32. Anti-HIV-1 activity of IL-32 was cancelled by M-CSF. Such effect of M-CSF was not seen with IL-32-induced M1-like features including high cytokine/chemokine production and CD80 expression. Of interest, IL-32-treated MF showed also M2-like features including high phagocytosis and expression of CD14 and CD163, the latter of which was up-regulated by combination with M-CSF. Our findings help to further understand the mechanisms regulating HIV-1 replication in MF, and the survival and M1/M2 ratio of MF.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015 2014 Other

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Myeloproliferative leukemia protein activation directly induces fibrocyte differentiation to cause myelofibrosis2017

    • Author(s)
      Maekawa T, Osawa Y, Izumi T, Nagao S, Takano K, Okada Y, Tachi N, Teramoto M, Kawamura T, Horiuchi T, Saga R, Kato S, Yamamura T, Watanabe J, Kobayashi A, Kobayashi S, Sato K, Hashimoto M, Suzu S, Kimura F
    • Journal Title

      Leukemia

      Volume: 31 Issue: 12 Pages: 2709

    • DOI

      10.1038/leu.2017.112

    • URL

      https://pure.teikyo.jp/en/publications/6d8778a4-b342-48d9-ab47-e24a6e3f81d7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential role of the formation of tunneling nanotubes in HIV-1 spread in macrophages.2016

    • Author(s)
      Hashimoto M, Bhuyan F, Hiyoshi M, Noyori O, Nasser H, Miyazaki M, Saito T, Kondoh Y, Osada H, Kimura S, Hase K, Ohno H, Suzu S
    • Journal Title

      J Immunol

      Volume: 196 Issue: 4 Pages: 1832-1841

    • DOI

      10.4049/jimmunol.1500845

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fibrocytes differ from macrophages but can be infected with HIV-1.2015

    • Author(s)
      M. Hashimoto, H. Nasser, F. Bhuyan, N. Kuse, Y. Satou, S. Harada, K. Yoshimura, J. Sakuragi, K. Monde, Y. Maeda, S. Welbourn, K. Strebel, E.W.A. El-Wahab, M. Miyazaki , S. Hattori, N. Chutiwitoonchai, M. Hiyoshi, S. Oka, M. Takiguchi, S. Suzu.
    • Journal Title

      The Journal of Immunology

      Volume: 195 Issue: 9 Pages: 4341-4350

    • DOI

      10.4049/jimmunol.1500955

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] M-CSF Inhibits Anti–HIV-1 Activity of IL-32, but They Enhance M2-like Phenotypes of Macrophages2014

    • Author(s)
      Abu Osman, Farzana Bhuyan, Michihiro Hashimoto, Hesham Nasser, Takaaki Maekawa, and Shinya Suzu
    • Journal Title

      The Journal of Immunology

      Volume: 192 Issue: 11 Pages: 5083-5089

    • DOI

      10.4049/jimmunol.1302732

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Susceptibility of circulating fibrocytes to HIV-1 in vitro and in vivo2016

    • Author(s)
      Suzu S
    • Organizer
      Keystone Symposia
    • Place of Presentation
      米国カリフォルニア
    • Year and Date
      2016-03-20
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] Research

    • URL

      http://www.caids.kumamoto-u.ac.jp/data/suzu/publication.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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