| Project/Area Number |
26461409
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
Shikama Yayoi 福島県立医科大学, 医学部, 教授 (40291562)
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| Co-Investigator(Kenkyū-buntansha) |
七島 勉 福島県立医科大学, 医学部, 博士研究員 (10192105)
野地 秀義 福島県立医科大学, 医学部, 准教授 (20347214)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 骨髄異形成症候群 / 好中球 / c-Fos / miR-34a / miR-155 / TNF-alpha / migration / 酸化ストレス / MDS / NF-kB / 遊走能 / TNFalpha / NF-ｋappaB / ＥＲストレス |
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| Outline of Final Research Achievements |
We found that c-Fos was reduced via overexpression of miR-34a and miR-155 in neutrophils isolated from patients with myelodysplastic syndromes (MDS). The reduction of c-Fos, which inhibited binding of NF-kB p60 to the promotor region of TNF-alpha DNA, induced overexpression of TNF-alpha under inflammation or oxidative stress (Shikama et al, PLoS One, 2016). The overexpression of miR-34a and miR-155 interfered with expression of Cdc42-specific guanine nucleotide exchange factors DOCK8 and FGD4, and a Rho family member Rac1, resulting in impairment in migration of MDS neutrophils toward fMLP and IL-8 (Cao et al, J Immunol, 2017).
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