Molecular mechanisms of ineffective hematopoiesis in myelodysplastic syndromes
Project/Area Number |
26461409
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Fukushima Medical University |
Principal Investigator |
Shikama Yayoi 福島県立医科大学, 医学部, 教授 (40291562)
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Co-Investigator(Kenkyū-buntansha) |
七島 勉 福島県立医科大学, 医学部, 博士研究員 (10192105)
野地 秀義 福島県立医科大学, 医学部, 准教授 (20347214)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 骨髄異形成症候群 / 好中球 / c-Fos / miR-34a / miR-155 / TNF-alpha / migration / 酸化ストレス / MDS / NF-kB / 遊走能 / TNFalpha / NF-kappaB / ERストレス |
Outline of Final Research Achievements |
We found that c-Fos was reduced via overexpression of miR-34a and miR-155 in neutrophils isolated from patients with myelodysplastic syndromes (MDS). The reduction of c-Fos, which inhibited binding of NF-kB p60 to the promotor region of TNF-alpha DNA, induced overexpression of TNF-alpha under inflammation or oxidative stress (Shikama et al, PLoS One, 2016). The overexpression of miR-34a and miR-155 interfered with expression of Cdc42-specific guanine nucleotide exchange factors DOCK8 and FGD4, and a Rho family member Rac1, resulting in impairment in migration of MDS neutrophils toward fMLP and IL-8 (Cao et al, J Immunol, 2017).
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Reduction of c-Fos via Overexpression of miR-34a Results in Enhancement of TNF- Production by LPS in Neutrophils from Myelodysplastic Syndrome Patients.2016
Author(s)
Shikama Y, Cao M, Ono T, Feng X, Noji H, Kimura H, Ogawa K, Suzuki Y, Ikeda K, Takeishi Y, Kimura J.
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Journal Title
PLoS One
Volume: 11
Issue: 8
Pages: e0158527-e0158527
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Excessive TNF-alpha production resulting from reduction of c-fos via aberrant expression of miR-34a in neutrophils from myelodysplastic syndrome patients.2015
Author(s)
Yayoi Shikama, Maiwan Cao, Tomoyuki Ono, Kojun Yamakawa, Michiko Anzai, Xiaomin Feng, Kazuko Kuriyama, Shun Hiruta, Hideyoshi Noji, Hideo Kimura, Kazuei Ogawa, Kazuhiko Ikeda, Yasuchika Takeishi, Junko Kimura
Organizer
57th ASH Annual Meeting and Esposition
Place of Presentation
Orland, USA
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] Excessive TNFalpha production via reduced expression of c-Fos in neutrophils from myelodysplastic syndrome patients.2015
Author(s)
Yayoi Shikama, Meiwan Cao, Kojun Yamakawa, Xiaomin Feng, Michiko Anzai, Tomoyuki Ono, Hideyoshi Noji, Kazuei Ogawa, Yuko Suzuki, Hideo Kimura, Yasuchika Takeishi, Junko Kimura
Organizer
第77回日本血液学会学術集会
Place of Presentation
金沢市
Year and Date
2015-10-17
Related Report
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