| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Among many players in the epigenetic regulation, the SWI/SNF ATP-dependent chromatin remodeling factor BRG1 has recently been demonstrated to be essential for leukemic stem cell (LSC) maintenance. Whereas BRG1 is implicated in the pathogenesis of Fanconi anemia and bone marrow failure, BRM, the homologue of BRG1, is expressed more specifically in HSCs compared with BRG1 and appeared to be involved in physiological regulation of HSCs. We have demonstrated an essential role of BRM in the maintenance of HSCs using genetically modified BRM-null mice. BRM-null HSCs showed profoundly impaired reconstitution capacity in competitive BMT assays and exhibited more activate cell cycling than wild type HSCs after bone marrow transplantation, suggesting that BRM plays a role in reversion of cycling HSCs into a quiescent state.
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