The role of integrin alpha 8 in the pathogenesis of multiple myeloma

• Project/Area Number: 26461420
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Osaka University
• Principal Investigator: ICHII Michiko 大阪大学, 医学系研究科, 寄附講座助教 (30633010)
• Co-Investigator(Kenkyū-buntansha): 金倉 譲 大阪大学, 医学系研究科, 教授 (20177489) ; 加藤 恒 大阪大学, 医学系研究科, 助教 (20705214) ; 横田 貴史 大阪大学, 医学系研究科, 助教 (60403200) ; 織谷 健司 大阪大学, 医学系研究科, 准教授 (70324762) ; 田所 誠司 大阪大学, 医学系研究科, 助教 (80403062)
• Co-Investigator(Renkei-kenkyūsha): YOKOSAKI Yasuyuki 広島大学, 保健管理センター, 准教授 (80210607)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨髄腫 / インテグリン / 多発性骨髄腫

Outline of Final Research Achievements

The purpose of this study is evaluating the role of integrin alpha 8 in the pathogenesis of multiple myeloma. It is reported that human plasma cells express integrin alpha 8. First, we analyzed the expression of integrin alpha 8 on myeloma cells. As a result, not all of myeloma cell lines express integrin alpha 8. In contrast, the expression of integrin alpha 8 was detected in myeloma cells derived from patient bone marrow and peripheral blood. The effect of integrin alpha 8 on proliferation and resistance against cytotoxic drugs seemed similar between integrin alpha 8 positive and negative myeloma cell lines. These results indicated that integrin alpha 8 might be useful for evaluating residual disease during treatment.

Research Products

• [Journal Article] Genetic abnormalities associated with acute lymphoblastic leukemia (2016)
  • Author(s): Yokota T, Kanakura Y
  • Journal Title: Cancer Science Volume: in press Issue: 6 Pages: 721-725
  • DOI: 10.1111/cas.12927
  • Peer Reviewed / Open Access
• [Journal Article] Human CalDAG-GEFI deficiency increases bleeding and delays αIIbβ3 activation. (2016)
  • Author(s): Kato H, Nakazawa Y, Kurokawa Y, Kashiwagi H, Morikawa Y, Morita D, Banno F, Honda S, Kanakura Y, Tomiyama Y.
  • Journal Title: Blood Volume: 128 Issue: 23 Pages: 2729-2733
  • DOI: 10.1182/blood-2016-03-704825
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias. (2016)
  • Author(s): Ishibashi T, Yokota T, Tanaka H, Ichii M, Sudo T, Satoh Y, Doi Y, Ueda T, Tanimura A, Hamanaka Y, Ezoe S, Shibayama H, Oritani K, Kanakura Y.
  • Journal Title: Experimental Hematology Volume: 44 Issue: 4 Pages: 269-281
  • DOI: 10.1016/j.exphem.2015.12.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Endothelial cell-selective adhesion molecule expression in hematopoietic stem/progenitor cells is essential for erythropoiesis recovery after bone marrow injury. (2016)
  • Author(s): Sudo T, Yokota T, Okuzaki D, Ueda T, Ichii M, Ishibashi T, Isono T, Habuchi Y, Oritani K, Kanakura Y.
  • Journal Title: PLoS One Volume: 11 Issue: 4 Pages: e0154189-e0154189
  • DOI: 10.1371/journal.pone.0154189
  • Peer Reviewed / Open Access
• [Journal Article] Estrogen-inducible sFRP5 inhibits early B-lymphopoiesis in vivo, but not during pregnancy (2015)
  • Author(s): Takafumi Yokota, Kenji Oritani, Takao Sudo, Tomohiko Ishibashi1, Yukiko Doi, Yoko Habuchi, Michiko Ichii, Kentaro Fukushima, Daisuke Okuzaki, Kazuma Tomizuka, Kengo Yamawaki, Makoto Kakitani, Akihiko Shimono, Eiichi Morii, Paul W. Kincade, Yuzuru Kanakura
  • Journal Title: European Journal of Immunology Volume: in press Issue: 5 Pages: 1390-1401
  • DOI: 10.1002/eji.201444939
  • Peer Reviewed / Open Access
• [Journal Article] Identification of osteoblast stimulating factor 5 as a negative regulator in the B-lymphopoietic niche. (2015)
  • Author(s): Fujita N, Ichii M, Maeda T, Saitoh N, Yokota T, Yamawaki K, Kakitani M, Tomizuka K, Oritani K, Kanakura Y
  • Journal Title: Experimental Hematology Volume: 43 Issue: 11 Pages: 963-973
  • DOI: 10.1016/j.exphem.2015.07.002
  • Peer Reviewed
• [Journal Article] Signal-transducing adaptor protein-2 regulates macrophage migration into inflammatory sites during dextran sodium sulfate induced colitis. (2014)
  • Author(s): Fujita N, Oritani K, Ichii M, Yokota T, Saitoh N, Okuzaki D, Sekine Y, Kon S, Muromoto R, Saitoh K, Yoshimura A, Matsuda T, Kanakura Y.
  • Journal Title: Eur J Immunol. Volume: 44 Issue: 6 Pages: 1791-1801
  • DOI: 10.1002/eji.201344239
  • Peer Reviewed / Open Access
• [Journal Article] Early B lymphocyte development: Similarities and differences in human and mouse. (2014)
  • Author(s): Ichii M, Oritani K, Kanakura Y.
  • Journal Title: World J Stem Cells. Volume: 6 Issue: 4 Pages: 421-431
  • DOI: 10.4252/wjsc.v6.i4.421
  • Peer Reviewed / Open Access
• [Journal Article] 多発性骨髄腫のimmunophenotyping (2014)
  • Author(s): 一井倫子、織谷健司、金倉譲
  • Journal Title: 臨床検査 Volume: 58 Pages: 1592-1597
• [Presentation] SATB1 Expression Helps in Identification of the Lymphoid-Lineage-Biased Trajectory of Func-tionally Fluctuating Hematopoietic Stem Cells (2016)
  • Author(s): Doi Y, Yokota T, Satoh Y, Ueda T, Shingai Y, Ichii M, Tanimura A, Ezoe S, Shibayama H, Oritani K,
  • Organizer: The American Society of Hematology 58th Annual Meeting
  • Place of Presentation: SanDiego convention center, SanDiego, CA, USA
  • Year and Date: 2016-12-03
• [Presentation] Anamorsin is essential for B-cell terminal differentiation. (2016)
  • Author(s): Hamanaka Y, Shibayama H, Tanimura A, Yokota T, Ezoe S, Ichii M, Ishibashi T, Doi Y, Nagate Y, Oritani K, Kanakura Y
  • Organizer: 21st Congress of the European Hematology Association
  • Place of Presentation: Bella Center, Copenhagen, Denmark
  • Year and Date: 2016-06-09
• [Presentation] An IMPDH Inhibitor, FF-10501 Possesses Activating Effects on Myeloid Differentiation as well as Anti-proliferation. (2015)
  • Author(s): Ichii M, Murase M, Komatsu K, Saito M, Toda J, Oritani K, Iwamura H, Kanakura Y.
  • Organizer: The American Society of Hematology 57th Annual Meeting
  • Place of Presentation: Orlando, FL, USA
  • Year and Date: 2015-12-07
  • Int'l Joint Research
• [Presentation] Endothelial cell-selective adhesion molecule is a novel human hematopoietic stem cell marker associated with a subset of human leukemias. (2015)
  • Author(s): Ishibashi T,Yokota T, Tanaka H, Ichii M, Sudo T, Satoh Y, Doi Y, Tanimura A, Hamanaka Y, Ezoe S, Shibayama H, Oritani K, Kanakura Y.
  • Organizer: The 44th Annual Scientific Meeting of International Society of Exoerimental Hematology
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2015-09-18
  • Int'l Joint Research
• [Presentation] An Anti-Apoptotic Molecule, Anamorsin, Is Upregulated in Anemia and Essential for Imperative Erythropoiesis. (2015)
  • Author(s): Tanimura A, Shibayama H, Hamanaka Y, Fujita N, Nagate Y, Ishibashi T, Ichii M, Yokota T, Ezoe S, Oritani K, Kanakura Y.
  • Organizer: JSH International Symposium 2015
  • Place of Presentation: 軽井沢プリンスホテル(長野県北佐久郡）
  • Year and Date: 2015-05-22
  • Int'l Joint Research
• [Presentation] REGULATION OF B LYMPHOPOIESIS BY SIGNAL-TRANSDUCING ADAPTOR PROTEIN-2, STAP-2 (2014)
  • Author(s): Ichii M, Oritani K, Fujita N, Saitoh N, Sekine Y, Muromoto R, Kon S, Saitoh K, Matsuda T, Kanakura Y
  • Organizer: 19th Congress of the European Hematology Association
  • Place of Presentation: Milan, Italy
  • Year and Date: 2014-06-13