| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Reconstitution of tissue resident macrophages and inflammatory macrophages after allogeneic hematopoietic stem cell transplantation (SCT) were assessed using murine model of allogeneic bone marrow transplantation (BMT). We found that severe GVHD promoted replacement of tissue resident macrophages in the target organs by donor-derived macrophages on day +14 after allogeneic BMT. Contrary, it took at least a few months for reconstitution of donor macrophages after syngeneic BMT, suggesting that allogeneic response accelerated donor reconstitution of tissue resident macrophages. Next, we addressed turnover of inflammatory macrophages after SCT. Massive infiltration of inflammatory macrophages was found in the lung and skin, and depletion of these cells using monoclonal antibodies ameliorated graft versus host disease (GVHD) after allogeneic SCT. Our findings have important clinical implications with respect to establishment of tolerance and pathophysiology of GVHD after allogeneic SCT.
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