Multiple developmental origins of adult blood cell lineages and its relevance to functions

• Project/Area Number: 26461442
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Mie University
• Principal Investigator: YAMANE Toshiyuki 三重大学, 医学系研究科, 准教授 (30452220)
• Co-Investigator(Kenkyū-buntansha): 山崎 英俊 三重大学, 医学系研究科, 教授 (00283987)
• Research Collaborator: ITO Chie
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 造血発生 / 細胞分化 / 造血幹細胞 / 発生 / 卵黄嚢 / マクロファージ / NK細胞 / B-1 B細胞 / B細胞 / B-1B細胞

Outline of Final Research Achievements

In recent years, it has been shown that some mature blood cells originated from embryonic stages persist in adult bodies independently from hematopoietic stem cells (HSC). In this study, we searched the origins of these HSC-independent cell lineages and examined the functional differences between embryo-derived cells and HSC-derived cells. Our study indicated the origin of some macrophages and NK cells in the yolk sac. In addition, we observed a functional differences between yolk sac-derived and adult HSC-derived NK cell lineages.

Research Products

• [Journal Article] Repression of Primitive Erythroid Program Is Critical for the Initiation of Multi-Lineage Hematopoiesis in Mouse Development (2017)
  • Author(s): Yamane T, Ito C, Washino A, Isono K, Yamazaki H
  • Journal Title: Journal of Cellular Physiology Volume: 232 Issue: 2 Pages: 323-330
  • DOI: 10.1002/jcp.25422
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Depletion of Neural Crest-Derived Cells Leads to Reduction in Plasma Noradrenaline and Alters B Lymphopoiesis (2017)
  • Author(s): Tsunokuma N, Yamane T, Matsumoto C, Tsuneto M, Isono K, Imanaka-Yoshida K, Yamazaki H
  • Journal Title: Journal of Immunology Volume: 198 Issue: 1 Pages: 156-169
  • DOI: 10.4049/jimmunol.1502592
  • Peer Reviewed
• [Presentation] Characterization of embryonic day 9-derived B progenitors (2016)
  • Author(s): Toshiyuki Yamane, Hidetoshi Yamazaki
  • Organizer: 第45回 日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-07
• [Presentation] Initiation of multi-lineage hematopoiesis during mouse ontogeny (2016)
  • Author(s): Toshiyuki Yamane, Chie Ito, Hidetoshi Yamazaki
  • Organizer: 第78回 日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-13
• [Presentation] Cellular and genetic cues to multipotency during ontogeny (2015)
  • Author(s): Toshiyuki Yamane, Chie Ito, Hidetoshi Yamazaki
  • Organizer: 第77回 日本血液学会学術集会
  • Place of Presentation: ANAクラウンプラザホテル金沢（金沢）
  • Year and Date: 2015-10-17
• [Presentation] Cellular and genetic cues to multipotency during ontogeny (2014)
  • Author(s): Toshiyuki Yamane, Hidetoshi Yamazaki
  • Organizer: 第43回 日本免疫学会学術集会
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-11
• [Presentation] Roles of neural crest-derived cells in the mouse bone marrow and thymic hemato-lymphopoiesis (2014)
  • Author(s): Naoki Tsunokuma, Toshiyuki Yamane, Hidetoshi Yamazaki
  • Organizer: 第43回 日本免疫学会学術集会
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-11
• [Remarks] 三重大学大学院 医学系研究科 幹細胞発生学分野
  • URL: http://www.medic.mie-u.ac.jp/physiol_regener/