| Project/Area Number |
26461446
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | National Cardiovascular Center Research Institute (2016)
Osaka University (2014-2015) |
|---|
Principal Investigator |
Ishibashi Tomohiko 国立研究開発法人国立循環器病研究センター, 研究所, 上級研究員 (30722285)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
金倉 譲 大阪大学, 医学系研究科, 教授 (20177489)
横田 貴史 大阪大学, 医学系研究科, 助教 (60403200)
織谷 健司 大阪大学, 医学系研究科, 准教授 (70324762)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 造血幹細胞 |
|---|
| Outline of Final Research Achievements |
Reliable markers are essential to increase our understanding of the biological features of human hematopoietic stem cells (HSCs). We found that endothelial cell-selective adhesion molecule (ESAM) can be used to purify human HSCs from all the currently available sources (adult bone marrow [BM], mobilized peripheral blood, and cord blood [CB]). Multipotent colony-forming units and long-term hematopoietic-reconstituting cells in immunodeficient mice were found exclusively in the ESAM(High) fraction of CD34+CD38- cells. The CD34+CD38- fraction of CB contained cells exhibiting extremely high expression of ESAM; these cells are likely to be related to the endothelial lineage. High ESAM expression was observed in some primary acute myeloid leukemia cells. Furthermore, KG-1a myeloid leukemia cells switched from ESAM negative to ESAM positive with repeated leukemia reconstitution in vivo. Thus, ESAM is a useful marker for studying both human HSCs and leukemia cells.
|
