ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias

• Project/Area Number: 26461446
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: National Cardiovascular Center Research Institute (2016); Osaka University (2014-2015)
• Principal Investigator: Ishibashi Tomohiko 国立研究開発法人国立循環器病研究センター, 研究所, 上級研究員 (30722285)
• Co-Investigator(Kenkyū-buntansha): 金倉 譲 大阪大学, 医学系研究科, 教授 (20177489) ; 横田 貴史 大阪大学, 医学系研究科, 助教 (60403200) ; 織谷 健司 大阪大学, 医学系研究科, 准教授 (70324762)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 造血幹細胞

Outline of Final Research Achievements

Reliable markers are essential to increase our understanding of the biological features of human hematopoietic stem cells (HSCs). We found that endothelial cell-selective adhesion molecule (ESAM) can be used to purify human HSCs from all the currently available sources (adult bone marrow [BM], mobilized peripheral blood, and cord blood [CB]). Multipotent colony-forming units and long-term hematopoietic-reconstituting cells in immunodeficient mice were found exclusively in the ESAM(High) fraction of CD34+CD38- cells. The CD34+CD38- fraction of CB contained cells exhibiting extremely high expression of ESAM; these cells are likely to be related to the endothelial lineage. High ESAM expression was observed in some primary acute myeloid leukemia cells. Furthermore, KG-1a myeloid leukemia cells switched from ESAM negative to ESAM positive with repeated leukemia reconstitution in vivo. Thus, ESAM is a useful marker for studying both human HSCs and leukemia cells.

Research Products

• [Journal Article] Endothelial cell-selective adhesion molecule expression in hematopoietic stem/progenitor cells is essential for erythropoiesis recovery after bone marrow injury. (2016)
  • Author(s): Sudo T, Yokota T, Okuzaki D, Ueda T, Ichii M, Ishibashi T, Isono T, Habuchi Y, Oritani K, Kanakura Y.
  • Journal Title: PLoS One Volume: 11 Issue: 4 Pages: e0154189-e0154189
  • DOI: 10.1371/journal.pone.0154189
  • Peer Reviewed / Open Access
• [Journal Article] ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias. (2016)
  • Author(s): Ishibashi T, Yokota T, Tanaka H, Ichii M, Sudo T, Satoh Y, Doi Y, Ueda T, Tanimura A, Hamanaka Y, Ezoe S, Shibayama H, Oritani K, Kanakura Y.
  • Journal Title: Experimental Hematology Volume: 44 Issue: 4 Pages: 269-281
  • DOI: 10.1016/j.exphem.2015.12.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Endothelial antigen ESAM is a human hematopoietic stem cell marker associated with a subset of human leukemias (2016)
  • Author(s): Tomohiko Ishibashi, Takafumi Yokota, Hirokazu Tanaka, Michiko Ichii, Yoshikazu Nakaoka, Yuzuru Kanakura
  • Organizer: 第24回日本血管生物医学会学術集会
  • Place of Presentation: 長崎ブリックホール
  • Year and Date: 2016-12-09
• [Presentation] ESAM is a human HSC marker associated with a subset of human leukemias (2016)
  • Author(s): Tomohiko Ishibashi, Takafumi Yokota, Hirokazu Tanaka, Michiko Ichii, Takao Sudo, Yusuke Satoh, Yukiko Doi, Tomoaki Ueda, Yasuhiro Shingai, Akira Tanimura, Yuri Hamanaka, Sachiko Ezoe, Hirohiko Shibayama, Kenji Oritani, Yuzuru Kanakura
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-13
• [Presentation] Endothelial antigen ESAM is a human HSC marker associated with a subset of human leukemias (2016)
  • Author(s): Tomohiko Ishibashi, Takafumi Yokota, Hirokazu Tanaka, Michiko Ichii, Takao Sudo, Yusuke Satoh, Yukiko Doi, Tomoaki Ueda, Akira Tanimura, Yuri Hamanaka, Sachiko Ezoe, Hirohiko Shibayama, Kenji Oritani, Yuzuru Kanakura
  • Organizer: 第14回幹細胞シンポジウム
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場
  • Year and Date: 2016-05-20
• [Presentation] ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias. (2015)
  • Author(s): Tomohiko Ishibashi, Takafumi Yokota, Hirokazu Tanaka, Michiko Ichii, Takao Sudo, Yusuke Satoh, Yukiko Doi, Akira Tanimura, Yuri Hamanaka, Sachiko Ezoe, Hirohiko Shibayama, Kenji Oritani, Yuzuru Kanakura
  • Organizer: 57th American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: Orange County Convention Center, Orlando, FL, USA
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] Endothelial cell-selective adhesion molecule is a novel human hematopoietic stem cell marker associated with a subset of human leukemias. (2015)
  • Author(s): Tomohiko Ishibashi, Takafumi Yokota, Hirokazu Tanaka, Michiko Ichii, Takao Sudo, Yusuke Satoh, Yukiko Doi, Akira Tanimura, Yuri Hamanaka, Sachiko Ezoe, Hirohiko Shibayama, Kenji Oritani, Yuzuru Kanakura
  • Organizer: International Society for Experimental Hematology 44th Annual Meeting
  • Place of Presentation: International Conference Center, Kyoto, Japan
  • Year and Date: 2015-09-18
  • Int'l Joint Research
• [Presentation] 血管内皮抗原ESAMは幹細胞ソースに関わらずヒト造血幹細胞のマーカーとなる (2014)
  • Author(s): 石橋知彦, 横田貴史, 一井倫子, 数藤孝雄, 佐藤友亮, 土居由貴子, 藤田奈津子, 長手泰宏, 濱中有理, 松井敬子, 谷村朗, 齋藤則充, 江副幸子, 柴山浩彦, 織谷健司, 金倉譲
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Year and Date: 2014-10-31