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Analysis of functions of a receptor tyrosine kinase selectively expressed in plasmacytoid dendritic cells.

Research Project

Project/Area Number 26461465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Collagenous pathology/Allergology
Research InstitutionKagawa University

Principal Investigator

Hoshino Katsuaki  香川大学, 医学部, 教授 (50324843)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords形質細胞様樹状細胞 / Ⅰ型インターフェロン / 全身性エリテマトーデス / I型インターフェロン
Outline of Final Research Achievements

We have demonstrated that an Ephrin receptor tyrosine kinase is selectively expressed in mouse plasmacytoid dendritic cells (pDCs) by use of a specific monoclonal antibody. When we incubate pDCs in vitro in the presence or absence of this monoclonal antibody, then stimulate with Toll-like receptor 7(TLR7) or TLR9 ligands, we have found that pDC produce comparable levels of cytokines including interferon-alpha. We have also found that pDCs normally developed in Flt3 ligand-induced bone marrow derived dendritic cells cultured in the presence of this monoclonal antibody. In order to analyze the function of the Ephrin receptor tyrosine kinase in mouse plasmacytoid dendritic cells, we have established two lines of knockout mouse. We are planning to analyze the phenotype of pDCs in these knockout mice.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (3 results) (of which Invited: 2 results)

  • [Journal Article] In vivo ablation of dendritic cell subset expressing the chemokine receptor XCR1.2016

    • Author(s)
      H. Hemmi, K. Hoshino, T. Kaisho.
    • Journal Title

      Methods Mol Biol.

      Volume: 1423 Pages: 247-53

    • DOI

      10.1007/978-1-4939-3606-9_17

    • ISBN
      9781493936045, 9781493936069
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crucial roles of XCR1-expressing dendritic cells and the XCR1-XCL1 chemokine axis in intestinal immune homeostasis.2016

    • Author(s)
      Ohta T, Sugiyama M, Hemmi H, Yamazaki C, Okura S, Sasaki I, Fukuda Y, Orimo T, Ishii KJ, Hoshino K, Ginhoux F, Kaisho T.
    • Journal Title

      Scientific Reports

      Volume: 162 Issue: 1 Pages: 23505-23505

    • DOI

      10.1038/srep23505

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node2016

    • Author(s)
      Kitano M., Yamazaki C., Takumi A., Ikeno T., Hemmi H., Takahashi N., Shimizu K., Fraser S. E., Hoshino K., Kaisho T. and Okada T.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 113 Issue: 4 Pages: 1044-1049

    • DOI

      10.1073/pnas.1513607113

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Antigen-specific cytotoxic T lymphocytes target airway CD103(+) and CD11b(+) dendritic cells to suppress allergic inflammation2016

    • Author(s)
      Daniels N. J., Hyde E., Ghosh S., Seo K., Price K. M., Hoshino K., Kaisho T., Okada T. and Ronchese F.
    • Journal Title

      Mucosal immunology

      Volume: 9 Issue: 1 Pages: 229-239

    • DOI

      10.1038/mi.2015.55

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Limitation of immune tolerance-inducing thymic epithelial cell development by Spi-B-mediated negative feedback regulation.2014

    • Author(s)
      Akiyama, N., Shinzawa, M., Miyauchi, M., Yanai, H., Tateishi, R., Shimo, Y., Ohshima, D., Matsuo, M., Sasaki. I., Hoshino, K., Wu, G., Yagi, S., Inoue, J., Kaisho, T. and Akiyama, T.
    • Journal Title

      J. Exp. Med.

      Volume: 211 Issue: 12 Pages: 2425-2438

    • DOI

      10.1084/jem.20141207

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-Cell Imaging of Caspase-1 Dynamics Reveals an All-or-None Inflammasome Signaling Response.2014

    • Author(s)
      Liu, T., Yamaguchi, Y., Shirasaki, Y., Shikada, K., Yamagishi, M., Hoshino, K., Kaisho, T., Takemoto, K., Suzuki, T., Kuranaga, E., Ohara, O., Miura, M.
    • Journal Title

      Cell Reports

      Volume: 8 Issue: 4 Pages: 974-982

    • DOI

      10.1016/j.celrep.2014.07.012

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 自然免疫受容体による樹状細胞活性化メカニズムの解析2017

    • Author(s)
      星野克明
    • Organizer
      2017肝免疫フォーラム
    • Place of Presentation
      芝パークホテル(東京都)
    • Year and Date
      2017-02-04
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 結核菌感染における初期免疫応答2015

    • Author(s)
      財賀大行、星野克明
    • Organizer
      第14回 四国免疫フォーラム
    • Place of Presentation
      愛媛大学医学部(愛媛県・松山市)
    • Year and Date
      2015-06-20
    • Related Report
      2015 Research-status Report
  • [Presentation] 教育講演1-自然免疫受容体による樹状細胞活性化メカニズムの解析-2014

    • Author(s)
      星野克明
    • Organizer
      第66回 日本皮膚科学会西部支部学術大会
    • Place of Presentation
      高松市
    • Year and Date
      2014-11-08
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2018-03-22  

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