Research Project
Grant-in-Aid for Scientific Research (C)
We have demonstrated that an Ephrin receptor tyrosine kinase is selectively expressed in mouse plasmacytoid dendritic cells (pDCs) by use of a specific monoclonal antibody. When we incubate pDCs in vitro in the presence or absence of this monoclonal antibody, then stimulate with Toll-like receptor 7(TLR7) or TLR9 ligands, we have found that pDC produce comparable levels of cytokines including interferon-alpha. We have also found that pDCs normally developed in Flt3 ligand-induced bone marrow derived dendritic cells cultured in the presence of this monoclonal antibody. In order to analyze the function of the Ephrin receptor tyrosine kinase in mouse plasmacytoid dendritic cells, we have established two lines of knockout mouse. We are planning to analyze the phenotype of pDCs in these knockout mice.
All 2017 2016 2015 2014
All Journal Article (6 results) (of which Int'l Joint Research: 3 results, Peer Reviewed: 6 results, Open Access: 4 results, Acknowledgement Compliant: 4 results) Presentation (3 results) (of which Invited: 2 results)
Methods Mol Biol.
Volume: 1423 Pages: 247-53
10.1007/978-1-4939-3606-9_17
Scientific Reports
Volume: 162 Issue: 1 Pages: 23505-23505
10.1038/srep23505
Proceedings of the National Academy of Sciences of the United States of America
Volume: 113 Issue: 4 Pages: 1044-1049
10.1073/pnas.1513607113
Mucosal immunology
Volume: 9 Issue: 1 Pages: 229-239
10.1038/mi.2015.55
J. Exp. Med.
Volume: 211 Issue: 12 Pages: 2425-2438
10.1084/jem.20141207
Cell Reports
Volume: 8 Issue: 4 Pages: 974-982
10.1016/j.celrep.2014.07.012