Therapeutic study of rheumatoid arthritis by humanized anti-PAD4 antibody
| Project/Area Number |
26461470
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Nagoya City University |
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Principal Investigator |
KANAZAWA Satoshi 名古屋市立大学, 大学院医学研究科, 助教 (90347401)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Mamoru 横浜市立大学, 大学院生命医科学研究科, 教授 (60170784)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 関節リウマチ / 間質性肺炎 / PAD4 / 抗CCP抗体 / 炎症 / トランスレーショナルリサーチ / モデル動物 / 膠原病 / 病態モデル動物 / 動物 / 免疫学 |
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| Outline of Final Research Achievements |
Peptidylarginine deiminase 4 (PAD4) that catalyzes the conversion of protein arginine residues to citrulline residues in the presence of Ca2+ is likely to be involved in rheumatoid arthritis (RA), because anti cyclic citrullinated protein antibodies against its catalytic modified peptides are associated with onset of RA disease. However, it is still not clear whether PAD4, in particular, its catalytic activity directly affects onset of RA symptoms. To reveal this, we created that anti PAD4 monoclonal antibodies recognized catalytic cleft of PAD4. Antibodies were obtained by phage display technique to be combined with immunization in chick. They were introduced by intraperitoneal administration in RA model mouse, called D1CC mouse, resulted in reduction of onset of inflammatory arthritis. Anti PAD4 antibody reduces inflammatory arthritis in D1CC mouse. We therefore concluded that PAD4 played critical role in the pathogenesis of initial phase of RA.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression.2016
Author(s)
Kawaguchi M, Hara N, Bilim V, Koike H, Suzuki M, Kim TS, Gao N, Dong Y, Zhang S, Fujinawa Y, Yamamoto O, Ito H, Tomita Y, Naruse Y, Sakamaki A, Ishii Y, Tsuneyama K, Inoue M, Itoh J, Yasuda M, Sakata N, Jung CG, Kanazawa S, Akatsu H, Minato H, Nojima T, Asai K, Miura Y.
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Journal Title
BMC Cancer.
Volume: 16
Issue: 1
Pages: 805-805
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A novel quinone derived from 5-hydroxyindoleacetic acid reacts with protein: Possible participation of oxidation of serotonin and its metabolite in the development of atherosclerosis2016
Author(s)
Kato, Y., Oki, K., Suga, N., Ono, S., Ishisaka, A., Miura, Y., Kanazawa, S., Naito, M., Kitamoto, N., Kettle, A. J.
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Journal Title
Free Radic Biol Med
Volume: 101
Pages: 500-510
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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