Exploratory research for histamine production regulators using epigenetic analysis

• Project/Area Number: 26461482
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Tohoku University
• Principal Investigator: Kudo Hisaaki 東北大学, 東北メディカル・メガバンク機構, 助教 (80647678)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ヒスタミン / ヒスチジン脱炭酸酵素

Outline of Final Research Achievements

Transcription activity experiment by demethylation of murine mast cell line P815 was performed as an experiment for inducing histamine production and preparation of cell samples. It has been reported that when the P815 cell line is treated with the demethylating agent; 5-azacytidine, demethylation occurs in the promoter region and the transcriptional activation of the Hdc gene is promoted, and the same treatment is carried out and chromatin immunity Sample preparation for sedimentation (ChIP) analysis was performed. At this time, cDNA was synthesized from the sample prepared at the same time and the expression analysis was carried out. As a result, an increase in expression of the Hdc gene was shown as in the above report. In addition, primers were designed in the promoter region to be analyzed for ChIP and PCR was carried out, and amplification was confirmed at 15 out of 17 sites.

Research Products

• [Journal Article] バイオバンクにおける試料の品質管理 ― 東北メディカル・メガバンク計画での取り組み (2017)
  • Author(s): 工藤久智，寺川貴裕，山下理宇
  • Journal Title: ヒト疾患のデータベースとバイオバンク情報をどう使い、どう活かすか？ゲノム医療をどう実現するか？ 2017年 実験医学増刊 Volume: 35 Pages: 122-127
• [Journal Article] Adjustment of Cell-Type Composition Minimizes Systematic Bias in Blood DNA Methylation Profiles Derived by DNA Collection Protocols. (2015)
  • Author(s): Shiwa Y, Hachiya T, Furukawa R, Ohmomo H, Ono K, Kudo H, Hata J, Hozawa A, Iwasaki M, Matsuda K, Minegishi N, Satoh M, Tanno K, Yamaji T, Wakai K, Hitomi J, Kiyohara Y, Kubo M, Tanaka H, Tsugane S, Yamamoto M, Sobue K, Shimizu A.
  • Journal Title: PLoS ONE Volume: 6 Issue: 1 Pages: 8018-8018
  • DOI: 10.1371/journal.pone.0147519
  • Peer Reviewed / Open Access
• [Journal Article] Validation of multiple single nucleotide variation calls by additional exome analysis with a semiconductor sequencer to supplement data of whole-genome sequencing of a human population (2014)
  • Author(s): Motoike IN, Matsumoto M, Danjoh I, Katsuoka F, Kojima K, Nariai N, Sato Y, Yamaguchi-Kabata Y, Ito S, Kudo H, Nishijima I, Nishikawa S, Pan X, Saito R, Saito S, Saito T, Shirota M, Tsuda K, Yokozawa J, Igarashi K, Minegishi N, Tanabe O, Fuse N, Nagasaki M, Kinoshita K, Yasuda J, and Yamamoto M.
  • Journal Title: BMC Genomics Volume: 10 Issue: 1 Pages: 637-637
  • DOI: 10.1186/1471-2164-15-673
  • Peer Reviewed / Open Access
• [Presentation] Evaluation of Biobank Sample Correctness and Integrity in TMM Biobank (2017)
  • Author(s): Hisaaki Kudo
  • Organizer: ISBER2017 Annual Meeting and Exhibits
  • Int'l Joint Research
• [Presentation] ゲノムコホート研究を支えるバイオバンクの構築について (2017)
  • Author(s): 工藤久智
  • Organizer: NGS現場の会 第5回研究会
  • Invited
• [Presentation] Reliability of ID Management in a Japanese Population-Based Biobank (2016)
  • Author(s): Hisaaki Kudo, Ichiko Nishijima, Takahiro Terakawa, Takahiro Nobukuni, Noriko Ishida, Riu Yamashita, Yumi Yamaguchi-Kabata, Kaname Kojima, Sakae Saito, Soichi Ogishima, Fumiki Katsuoka, Masao Nagasaki, Jun Yasuda, Mamoru Satoh, Naoko Minegishi, Makoto Sasaki, Masayuki Yamamoto
  • Organizer: Europe Biobank Week 2016
  • Place of Presentation: オーストリア、ウィーン
  • Year and Date: 2016-09-13
  • Int'l Joint Research
• [Presentation] Assessment of ID Integrity with ABO blood typing from Questionnaire to Genomic Data in Japanese Biobank: ToMMo (2015)
  • Author(s): Hisaaki Kudo, Ichiko Nishijima, Takahiro Terakawa, Takahiro Nobukuni , Sakae Saito, Soichi Ogishima, Fumiki Katsuoka, Masao Nagasaki, Jun Yasuda, Naoko Minegishi, Masayuki Yamamoto
  • Organizer: ISBER 2015 Annual Meeting and Exhibits
  • Place of Presentation: アメリカ合衆国アリゾナ州フェニックス、シェラトンフェニックスダウンタウン
  • Year and Date: 2015-05-05
  • Int'l Joint Research
• [Presentation] バイオバンクにおけるメガ級（数百万本）のサンプル管理について (2014)
  • Author(s): 工藤久智
  • Organizer: 第３回生命医薬情報学連合大会
  • Place of Presentation: 宮城県仙台市 仙台国際センター
  • Year and Date: 2014-10-04
  • Invited