Exploratory research for histamine production regulators using epigenetic analysis
Project/Area Number |
26461482
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Collagenous pathology/Allergology
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Research Institution | Tohoku University |
Principal Investigator |
Kudo Hisaaki 東北大学, 東北メディカル・メガバンク機構, 助教 (80647678)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | ヒスタミン / ヒスチジン脱炭酸酵素 |
Outline of Final Research Achievements |
Transcription activity experiment by demethylation of murine mast cell line P815 was performed as an experiment for inducing histamine production and preparation of cell samples. It has been reported that when the P815 cell line is treated with the demethylating agent; 5-azacytidine, demethylation occurs in the promoter region and the transcriptional activation of the Hdc gene is promoted, and the same treatment is carried out and chromatin immunity Sample preparation for sedimentation (ChIP) analysis was performed. At this time, cDNA was synthesized from the sample prepared at the same time and the expression analysis was carried out. As a result, an increase in expression of the Hdc gene was shown as in the above report. In addition, primers were designed in the promoter region to be analyzed for ChIP and PCR was carried out, and amplification was confirmed at 15 out of 17 sites.
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Report
(5 results)
Research Products
(8 results)
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[Journal Article] Adjustment of Cell-Type Composition Minimizes Systematic Bias in Blood DNA Methylation Profiles Derived by DNA Collection Protocols.2015
Author(s)
Shiwa Y, Hachiya T, Furukawa R, Ohmomo H, Ono K, Kudo H, Hata J, Hozawa A, Iwasaki M, Matsuda K, Minegishi N, Satoh M, Tanno K, Yamaji T, Wakai K, Hitomi J, Kiyohara Y, Kubo M, Tanaka H, Tsugane S, Yamamoto M, Sobue K, Shimizu A.
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Journal Title
PLoS ONE
Volume: 6
Issue: 1
Pages: 8018-8018
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Validation of multiple single nucleotide variation calls by additional exome analysis with a semiconductor sequencer to supplement data of whole-genome sequencing of a human population2014
Author(s)
Motoike IN, Matsumoto M, Danjoh I, Katsuoka F, Kojima K, Nariai N, Sato Y, Yamaguchi-Kabata Y, Ito S, Kudo H, Nishijima I, Nishikawa S, Pan X, Saito R, Saito S, Saito T, Shirota M, Tsuda K, Yokozawa J, Igarashi K, Minegishi N, Tanabe O, Fuse N, Nagasaki M, Kinoshita K, Yasuda J, and Yamamoto M.
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Journal Title
BMC Genomics
Volume: 10
Issue: 1
Pages: 637-637
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Reliability of ID Management in a Japanese Population-Based Biobank2016
Author(s)
Hisaaki Kudo, Ichiko Nishijima, Takahiro Terakawa, Takahiro Nobukuni, Noriko Ishida, Riu Yamashita, Yumi Yamaguchi-Kabata, Kaname Kojima, Sakae Saito, Soichi Ogishima, Fumiki Katsuoka, Masao Nagasaki, Jun Yasuda, Mamoru Satoh, Naoko Minegishi, Makoto Sasaki, Masayuki Yamamoto
Organizer
Europe Biobank Week 2016
Place of Presentation
オーストリア、ウィーン
Year and Date
2016-09-13
Related Report
Int'l Joint Research
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[Presentation] Assessment of ID Integrity with ABO blood typing from Questionnaire to Genomic Data in Japanese Biobank: ToMMo2015
Author(s)
Hisaaki Kudo, Ichiko Nishijima, Takahiro Terakawa, Takahiro Nobukuni , Sakae Saito, Soichi Ogishima, Fumiki Katsuoka, Masao Nagasaki, Jun Yasuda, Naoko Minegishi, Masayuki Yamamoto
Organizer
ISBER 2015 Annual Meeting and Exhibits
Place of Presentation
アメリカ合衆国アリゾナ州フェニックス、シェラトンフェニックスダウンタウン
Year and Date
2015-05-05
Related Report
Int'l Joint Research
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