Inhibitory effect of allergic reaction by IL33 receptor ST2

• Project/Area Number: 26461494
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Juntendo University
• Principal Investigator: MAEDA Keiko 順天堂大学, 医学(系)研究科(研究院), 特任助教 (20053374)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: IL33受容体ST2 / アレルギー / マスト細胞 / ST2 / IL33 レセプター / アレルギー反応

Outline of Final Research Achievements

Soluble ST2, a spliced variant of IL33 receptor ST2, was released from lymphocytes of allergy or inflammatory diseases. It is reported that sST2 act as a decoy receptor for IL33 and modulates IL33 activity, and the level of sST2 in sera was elevated in heart failure and play as a biomarker. Although sST2 is elevated in sera of patients, the mechanisms of release from cells remains unclear. We have investigated how mouse mast cells release sST2 protein from cytosol under antigen stimulation. When mast cells were pretreated with protein synthesis inhibitor and degranulated, they released sST2 and granule, simultaneously. Immunofluorescent analysis showed that CD63, degranulation marker, and ST2 in cytosol are migrated to cell surface by antigen stimulation. These data suggested that sST2 protein is stored in granule and others, and release from cells by stimulation.

Research Products

• [Journal Article] Ceramide-CD300f Binding Inhibits Lipopolysaccharide-induced Skin Inflammation (2017)
  • Author(s): Shiba E, Izawa K, Kaitani A, Isobe M, Maehara A, Uchida K, Maeda K, Nakano N, Ogawa H, Okumura K, Kitamura T, Shimizu T and Kitaura J
  • Journal Title: J. Biol. Chem. Volume: 292 Pages: 2924-2932
  • Peer Reviewed / Open Access
• [Journal Article] Role of PU.1 in MHC class II Expression via CIITA Transcription in Plasmacytoid Dendritic Cells (2016)
  • Author(s): Miura R, Kasakura K, Nakano N, Hara M, Maeda K, Yashiro T, and Nisiyama C
  • Journal Title: PloS One Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of PU.1 in NFATc1 promoter function in osteoclast development, (2015)
  • Author(s): Ishiyama, K., Yashiro, T., Nakano, N., Kasakura, K., Miura, R., Hara, M., Kawai, F., Maeda, K., Tamura, N., Okumura, K., Ogawa, H., Takasaki, Y., Nishiyama, C.
  • Journal Title: Allergol. Int. Volume: 64 Pages: 241-247
  • NAID: 130005290298
  • Peer Reviewed / Open Access
• [Journal Article] The Transcription Factor Ehf Is Involved in TGF-β-Induced Suppression of FcεRI and c-Kit Expression and FcεRI-Mediated Activation in Mast Cells. (2015)
  • Author(s): Yamazaki S, Nakano N, Honjo A, Hara M, Maeda K, Nishiyama C, Kitaura J, Ohtsuka Y, Okumura K, Ogawa H, Shimizu T
  • Journal Title: Journal of immunology Volume: 195(7) Issue: 7 Pages: 3427-3435
  • DOI: 10.4049/jimmunol.1402856
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of PU.1 in NFATc1 promoter function in osteoclast development (2015)
  • Author(s): Ishiyama, K., Yashiro, T., Nakano, N., Kasakura, K., Miura, R., Hara, M., Kawai, F., Maeda, K., Tamura, N., Okumura, K., Ogawa, H., Takasaki, Y., Nishiyama, C.
  • Journal Title: Allergol. Int. Volume: in press
  • NAID: 130005290298
  • Peer Reviewed / Open Access / Acknowledgement Compliant