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Analysis of cell death of sputum neutrophils aiming at development of a diagnostic method for new pneumonia

Research Project

Project/Area Number 26461502
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Infectious disease medicine
Research InstitutionOsaka University

Principal Investigator

YOSHIDA HISAO  大阪大学, 医学部附属病院, 助教 (80598574)

Co-Investigator(Kenkyū-buntansha) 朝野 和典  大阪大学, 医学系研究科, 教授 (40202204)
明田 幸宏  大阪大学, 医学部附属病院, 講師 (60444527)
関 雅文  大阪大学, 医学部附属病院, 講師 (80432970)
萩谷 英大  大阪大学, 医学部附属病院, 助教 (30718531)
Research Collaborator TAKEGAWA RYOSUKE  大阪大学, 医学部附属病院, 医員
HAMAGUCHI SHIGETO  ニューヨーク市立大学, 研究員
YAMAMOTO NORIHISA  大阪大学, 医学部附属病院, 医員
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords肺炎 / 好中球 / グラム染色 / NETs / hyper spectral imaging
Outline of Final Research Achievements

The diagnosis of pneumonia is still difficult now. We observed Gram stained slides of sputum collected from patients with pneumonia for observation with an optical microscope using a dedicated camera that can analyze spectral data in detail. With this camera we were able to obtain data dividing the image in the neutrophils every 5 nm in the wavelength range of 400 to 1000 nm of light. By analyzing the obtained data with dedicated software, it is possible to distinguish differences in color tone that can not be discerned by human eyes. It was shown that the difference in color can be distinguished and classified as the difference in activation of neutrophils.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2016 2015

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Analysis of neutrophil by hyper spectral imaging -a preliminary report-2016

    • Author(s)
      Takegawa Ryosuke
    • Organizer
      36th International Symposium on Intensive Care and Emergency Medicine
    • Place of Presentation
      BRUSSELS MEETING CENTER
    • Year and Date
      2016-03-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] The presence of Neutrophil Extracellular Traps in bronchial aspirate of patient diagnosed as ARDS or acute exacerbation of idiopathic pulmonary fibrosis2015

    • Author(s)
      Masahiro Ojima
    • Organizer
      The International Symposium on Intensive Care and Emergency Medicine
    • Place of Presentation
      Belgium, Brussels
    • Year and Date
      2015-03-17 – 2015-03-20
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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