Development of useful method in differential diagnosis of Creutzfeldt-Jakob diseases

• Project/Area Number: 26461507
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: University of Miyazaki (2016); Nagasaki University (2014-2015)
• Principal Investigator: MORI Tsuyoshi 宮崎大学, 医学部, 助教 (40426565)
• Co-Investigator(Kenkyū-buntansha): 佐藤 克也 長崎大学, 医歯薬学総合研究科(保健学科), 教授 (70398147)
• Co-Investigator(Renkei-kenkyūsha): ATARASHI Ryuichiro 宮崎大学, 医学部, 教授 (90452846)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: パネルRT-QUIC法 / 孤発性CJD

Outline of Final Research Achievements

Sporadic Creutzfeldt-Jakob disease (sCJD), a majority of human prion diseases, is classified in different subtypes which contribute to the occurrence of distinct clinical-pathological phenotypes. In this study, we sought to determine whether a modification of real-time quaking-induced conversion (RT-QUIC) assay, a sensitive diagnostic test for prion diseases, allows for distinguish between subtypes of sCJD. We constructed a several different types of recombinant prion protein (recPrP) mutant as a substrate for RT-QUIC reaction. We compared the prion detective sensitivities of RT-QUIC reaction with mutant recPrPs. RT-QUICs were performed with brain homogenates from several types of prion disease patients or infected mice. As a result, there were slightly differences in RT-QUIC signals between specimens by using mutant recPrP as substrate.

Research Products

• [Journal Article] A direct assessment of human prion adhered to steel wire using real-time quaking-induced conversion. (2016)
  • Author(s): Mori T, Atarashi R, Furukawa K, Takatsuki H, Satoh K, Sano K, Nakagaki T, Ishibashi D, Ichimiya K, Hamada M, Nakayama T, Nishida N.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 24993-24993
  • DOI: 10.1038/srep24993
  • Peer Reviewed / Open Access
• [Journal Article] Sequential Washing with Electrolyzed Alkaline and Acidic Water Effectively Removes Pathogens from Metal Surfaces. (2016)
  • Author(s): Nakano Y, Akamatsu N, Mori T, Sano K, Satoh K, Nagayasu T, Miyoshi Y, Sugio T, Sakai H, Sakae E, Ichimiya K, Hamada M, Nakayama T, Fujita Y, Yanagihara K, Nishida N. Sequential
  • Journal Title: PLoS One Volume: 11(5) Issue: 5 Pages: e0156058-e0156058
  • DOI: 10.1371/journal.pone.0156058
  • Peer Reviewed / Open Access
• [Journal Article] Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients. (2016)
  • Author(s): Takatsuki H, Fuse T, Nakagaki T, Mori T, Mihara B, Takao M, Iwasaki Y, Yoshida M, Murayama S, Atarashi R, Nishida N, Satoh K.
  • Journal Title: EBioMedicine. Volume: 12 Pages: 150-155
  • DOI: 10.1016/j.ebiom.2016.08.033
  • NAID: 120006987618
  • Peer Reviewed / Open Access
• [Presentation] Wire-QuIC reaction can detect abnormal human prion seeds from contaminated stainless steel-wire (2016)
  • Author(s): Tsuyoshi Mori, Ryuichiro Atarashi, Katsuya Satoh, Daisuke Ishibashi, Takehiro Nakagaki, Noriyuki Nishida
  • Organizer: The 64th Annual Meeting of the Japanese Society for Virology
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-10-23
• [Presentation] "Wire-QuIC": a new detection system of human prion (2016)
  • Author(s): Tsuyoshi Mori, Ryuichiro Atarashi, Hanae Takatsuki, Katsuya Satoh, Takehiro Nakagaki, Daisuke Ishibashi, Noriyuki Nishida
  • Organizer: PRION 2016 TOKYO
  • Place of Presentation: Hitotsubashi Hall, National Center of Sciences Building
  • Year and Date: 2016-05-10
  • Int'l Joint Research